LS-C354065

antibody from LSBio

Targeting: MAPK6 ERK3, HsT17250, p97MAPK, PRKM6

Western blot (Enhanced validation)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C354065

Provider

LSBio

Product name

MAPK6 / ERK3 Antibody (Internal) LS-C354065

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human, Mouse, Bovine, Chicken/Avian, Simian

Host

Rabbit

Storage

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of ERK3 expression in HeLa (A); NIH3T3 (B) whole cell lysates.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemical analysis of ERK3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemical analysis of ERK3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.