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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PA1-31991 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-FGFR4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-31993 detects DDR2 from human samples. PA1-31993 has been successfully used in ELISA and Western blot applications. The PA1-31993 immunogen is synthetic peptide CYFRSEASEWEPNAISF conjugated to KLH, corresponding to amino acids 304-320 of Human DDR2 (Accession Q16832). Suggested postive control is HL60 cell lysate
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.25 mg/ml
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of FGFR4/CD334 in HeLa cells using a FGFR4/CD334 polyclonal antibody (Product # PA1-31991). Cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min) and incubated with primary antibody ata dilution of 1:200 for 2 hrs at room temperature. Detection was performed using an Alexa Fluor 488 conjugated donkey anti-rabbit secondary antibody (green) at a dilution of 1:1000 for 1 hr. Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of FGFR4/CD334 in formalin-fixed, paraffin-embedded human cancer tissue using a FGFR4/CD334 polyclonal antibody (Product # PA1-31991) peroxidase-conjugated to the secondary antibody, followed by AEC staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of FGFR4/CD334 in WiDr cells (bottom histogram) compared to a negative control cell (top histogram) using a FGFR4/CD334 polyclonal antibody (Product # PA1-31991). Secondary detection was performed using a FITC-conjugated goat-anti-rabbit secondary antibody.
