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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- GTX25481 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX25481, RRID:AB_380554
- Product name
- FGFR4 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- FGFR4 Antibody (E39) (GTX25481) western blot analysis in 293 cell line lysates (35μg/lane). This demonstrates the FGFR4 antibody detected the FGFR4 protein (arrow).
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Fluorescent confocal image of HeLa cells stained with FGFR4 (N-term) antibody (GTX25481). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with FGFR4 (N-term) primary antibody (GTX25481) (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor? 488 conjμgated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).?
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary FGFR4 Antibody (E39) (GTX25481), which was peroxidase-conjμgated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.HC = hepatocarcinoma.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric analysis of WiDr cells using FGFR4 Antibody (N-term) (GTX25481) (bottom histogram) compared to a negative control (top histogram). FITC-conjμgated goat-anti-rabbit secondary antibodies were used for the analysis.
