Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-105531 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-FGFR4 (Tyr642) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Antibody detects endogenous levels of FGFR4 only when phosphorylated at Tyr642.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Gait disturbances and muscle dysfunction in fibroblast growth factor 2 knockout mice.
Homer-Bouthiette C, Xiao L, Hurley MM
Scientific reports 2021 May 26;11(1):11005
Scientific reports 2021 May 26;11(1):11005
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-FGFR4 (Tyr642) in Jurkat (Lane 1: treated with phospho-blocking peptide; Lane 2: treated with non-phospho-blocking peptide). Samples were incubated with Phospho-FGFR4 (Tyr642) polyclonal antibody (Product # PA5-105531).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of paraffin-embedded Phospho-FGFR4 (Tyr642) in rat lung tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-FGFR4 (Tyr642) polyclonal antibody (Product # PA5-105531) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 8 Expression of FGFR protein and MAPK/ERK signaling in skeletal muscle from WT and Fgf2KO mice. Young (5 m) and old (20 m) WT and Fgf2KO mouse triceps muscles were isolated and total protein extracted for western blotting. ( a ) phosphoFGFR1 Western blot and ( b ) quantification. ( c ) phosphoFGFR3 Western blot and ( d ) quantification. ( e ) phosphoFGFR4 Western blot and ( f ) quantification. ( g ) phosphoERK and total ERK Western blot and ( h ) quantification . ( a , b ) phosphoFGFR1; ( c , d ) phosphoFGFR3 were significantly increased, while ( e , f ) phosphoFGFR4 was significantly decreased in old muscle of both genotypes. ( g , h ) phosphoERK1/2 was significantly increased in 20 m old as well 5 m and 20 m Fgf2KO compared with 5 m WT. n = 3 mice/group. *Compared to corresponding WT p < 0.05, # compared to corresponding 5 m p < 0.05.