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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [21]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-20357 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-20357, RRID:AB_641107
- Product name
- Anti-GAPDH
- Antibody type
- Polyclonal (Antigen purified)
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Goat
Submitted references A novel chemically directed route for the generation of definitive endoderm from human embryonic stem cells based on inhibition of GSK-3.
Carbon monoxide stimulates global protein methylation via its inhibitory action on cystathionine β-synthase.
Induction of neutrophil gelatinase-associated lipocalin expression by co-stimulation with interleukin-17 and tumor necrosis factor-alpha is controlled by IkappaB-zeta but neither by C/EBP-beta nor C/EBP-delta.
BCL2 and CASP8 regulation by NF-kappaB differentially affect mitochondrial function and cell fate in antiestrogen-sensitive and -resistant breast cancer cells.
Age-dependent response of CCAAT/enhancer binding proteins following traumatic brain injury in mice.
Differential regulation of alpha7 nicotinic receptor gene (CHRNA7) expression in schizophrenic smokers.
Human mitochondrial RNA turnover caught in flagranti: involvement of hSuv3p helicase in RNA surveillance.
Chromosome 21-derived microRNAs provide an etiological basis for aberrant protein expression in human Down syndrome brains.
ASPM-associated stem cell proliferation is involved in malignant progression of gliomas and constitutes an attractive therapeutic target.
PKCalpha-induced drug resistance in pancreatic cancer cells is associated with transforming growth factor-beta1.
Nerve growth factor-regulated emergence of functional delta-opioid receptors.
Substance P increases cell-surface expression of CD74 (receptor for macrophage migration inhibitory factor): in vivo biotinylation of urothelial cell-surface proteins.
Dose-dependent transitions in Nrf2-mediated adaptive response and related stress responses to hypochlorous acid in mouse macrophages.
Fasting-induced hepatic production of DHEA is regulated by PGC-1alpha, ERRalpha, and HNF4alpha.
Cytoplasmic CUG RNA foci are insufficient to elicit key DM1 features.
Regulation of a novel alphaN-catenin splice variant in schizophrenic smokers.
SIRT3 is a stress-responsive deacetylase in cardiomyocytes that protects cells from stress-mediated cell death by deacetylation of Ku70.
Exacerbated glial response in the aged mouse hippocampus following controlled cortical impact injury.
Lapatinib (Tykerb, GW572016) reverses multidrug resistance in cancer cells by inhibiting the activity of ATP-binding cassette subfamily B member 1 and G member 2.
Perturbation of wild-type lamin A metabolism results in a progeroid phenotype.
Short hairpin RNA–expressing bacteria elicit RNA interference in mammals
Bone HK, Nelson AS, Goldring CE, Tosh D, Welham MJ
Journal of cell science 2011 Jun 15;124(Pt 12):1992-2000
Journal of cell science 2011 Jun 15;124(Pt 12):1992-2000
Carbon monoxide stimulates global protein methylation via its inhibitory action on cystathionine β-synthase.
Yamamoto T, Takano N, Ishiwata K, Suematsu M
Journal of clinical biochemistry and nutrition 2011 Jan;48(1):96-100
Journal of clinical biochemistry and nutrition 2011 Jan;48(1):96-100
Induction of neutrophil gelatinase-associated lipocalin expression by co-stimulation with interleukin-17 and tumor necrosis factor-alpha is controlled by IkappaB-zeta but neither by C/EBP-beta nor C/EBP-delta.
Karlsen JR, Borregaard N, Cowland JB
The Journal of biological chemistry 2010 May 7;285(19):14088-100
The Journal of biological chemistry 2010 May 7;285(19):14088-100
BCL2 and CASP8 regulation by NF-kappaB differentially affect mitochondrial function and cell fate in antiestrogen-sensitive and -resistant breast cancer cells.
Nehra R, Riggins RB, Shajahan AN, Zwart A, Crawford AC, Clarke R
The FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Jun;24(6):2040-55
The FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Jun;24(6):2040-55
Age-dependent response of CCAAT/enhancer binding proteins following traumatic brain injury in mice.
Sandhir R, Berman NE
Neurochemistry international 2010 Jan;56(1):188-93
Neurochemistry international 2010 Jan;56(1):188-93
Differential regulation of alpha7 nicotinic receptor gene (CHRNA7) expression in schizophrenic smokers.
Mexal S, Berger R, Logel J, Ross RG, Freedman R, Leonard S
Journal of molecular neuroscience : MN 2010 Jan;40(1-2):185-95
Journal of molecular neuroscience : MN 2010 Jan;40(1-2):185-95
Human mitochondrial RNA turnover caught in flagranti: involvement of hSuv3p helicase in RNA surveillance.
Szczesny RJ, Borowski LS, Brzezniak LK, Dmochowska A, Gewartowski K, Bartnik E, Stepien PP
Nucleic acids research 2010 Jan;38(1):279-98
Nucleic acids research 2010 Jan;38(1):279-98
Chromosome 21-derived microRNAs provide an etiological basis for aberrant protein expression in human Down syndrome brains.
Kuhn DE, Nuovo GJ, Terry AV Jr, Martin MM, Malana GE, Sansom SE, Pleister AP, Beck WD, Head E, Feldman DS, Elton TS
The Journal of biological chemistry 2010 Jan 8;285(2):1529-43
The Journal of biological chemistry 2010 Jan 8;285(2):1529-43
ASPM-associated stem cell proliferation is involved in malignant progression of gliomas and constitutes an attractive therapeutic target.
Bikeye SN, Colin C, Marie Y, Vampouille R, Ravassard P, Rousseau A, Boisselier B, Idbaih A, Calvo CF, Leuraud P, Lassalle M, El Hallani S, Delattre JY, Sanson M
Cancer cell international 2010 Jan 11;10:1
Cancer cell international 2010 Jan 11;10:1
PKCalpha-induced drug resistance in pancreatic cancer cells is associated with transforming growth factor-beta1.
Chen Y, Yu G, Yu D, Zhu M
Journal of experimental & clinical cancer research : CR 2010 Aug 5;29:104
Journal of experimental & clinical cancer research : CR 2010 Aug 5;29:104
Nerve growth factor-regulated emergence of functional delta-opioid receptors.
Bie B, Zhang Z, Cai YQ, Zhu W, Zhang Y, Dai J, Lowenstein CJ, Weinman EJ, Pan ZZ
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010 Apr 21;30(16):5617-28
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010 Apr 21;30(16):5617-28
Substance P increases cell-surface expression of CD74 (receptor for macrophage migration inhibitory factor): in vivo biotinylation of urothelial cell-surface proteins.
Meyer-Siegler KL, Xia SL, Vera PL
Mediators of inflammation 2009;2009:535348
Mediators of inflammation 2009;2009:535348
Dose-dependent transitions in Nrf2-mediated adaptive response and related stress responses to hypochlorous acid in mouse macrophages.
Woods CG, Fu J, Xue P, Hou Y, Pluta LJ, Yang L, Zhang Q, Thomas RS, Andersen ME, Pi J
Toxicology and applied pharmacology 2009 Jul 1;238(1):27-36
Toxicology and applied pharmacology 2009 Jul 1;238(1):27-36
Fasting-induced hepatic production of DHEA is regulated by PGC-1alpha, ERRalpha, and HNF4alpha.
Grasfeder LL, Gaillard S, Hammes SR, Ilkayeva O, Newgard CB, Hochberg RB, Dwyer MA, Chang CY, McDonnell DP
Molecular endocrinology (Baltimore, Md.) 2009 Aug;23(8):1171-82
Molecular endocrinology (Baltimore, Md.) 2009 Aug;23(8):1171-82
Cytoplasmic CUG RNA foci are insufficient to elicit key DM1 features.
Dansithong W, Wolf CM, Sarkar P, Paul S, Chiang A, Holt I, Morris GE, Branco D, Sherwood MC, Comai L, Berul CI, Reddy S
PloS one 2008;3(12):e3968
PloS one 2008;3(12):e3968
Regulation of a novel alphaN-catenin splice variant in schizophrenic smokers.
Mexal S, Berger R, Pearce L, Barton A, Logel J, Adams CE, Ross RG, Freedman R, Leonard S
American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics 2008 Sep 5;147B(6):759-68
American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics 2008 Sep 5;147B(6):759-68
SIRT3 is a stress-responsive deacetylase in cardiomyocytes that protects cells from stress-mediated cell death by deacetylation of Ku70.
Sundaresan NR, Samant SA, Pillai VB, Rajamohan SB, Gupta MP
Molecular and cellular biology 2008 Oct;28(20):6384-401
Molecular and cellular biology 2008 Oct;28(20):6384-401
Exacerbated glial response in the aged mouse hippocampus following controlled cortical impact injury.
Sandhir R, Onyszchuk G, Berman NE
Experimental neurology 2008 Oct;213(2):372-80
Experimental neurology 2008 Oct;213(2):372-80
Lapatinib (Tykerb, GW572016) reverses multidrug resistance in cancer cells by inhibiting the activity of ATP-binding cassette subfamily B member 1 and G member 2.
Dai CL, Tiwari AK, Wu CP, Su XD, Wang SR, Liu DG, Ashby CR Jr, Huang Y, Robey RW, Liang YJ, Chen LM, Shi CJ, Ambudkar SV, Chen ZS, Fu LW
Cancer research 2008 Oct 1;68(19):7905-14
Cancer research 2008 Oct 1;68(19):7905-14
Perturbation of wild-type lamin A metabolism results in a progeroid phenotype.
Candelario J, Sudhakar S, Navarro S, Reddy S, Comai L
Aging cell 2008 Jun;7(3):355-67
Aging cell 2008 Jun;7(3):355-67
Short hairpin RNA–expressing bacteria elicit RNA interference in mammals
Shuanglin Xiang, Johannes Fruehauf, Chiang J Li
Nature Biotechnology 2006 May;24(6):697-702
Nature Biotechnology 2006 May;24(6):697-702
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:2000
- Lane 1
- Marker [kDa]: 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 28
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human kidney shows cytoplasmic and nuclear positivity in cells in tubules.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
