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- Antibody Data
- Antigen structure
- References [15]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- 60004-1-Ig - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#60004-1-Ig, RRID:AB_2107436
- Product name
- GAPDH antibody
- Antibody type
- Monoclonal
- Description
- GAPDH antibody (Cat. #60004-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, mouse, rat, yeast, plant, zebrafish and has been validated for the following applications: FC, IF, IP, WB, ELISA.
- Reactivity
- Human, Mouse, Rat, Yeast, Zebrafish
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 1E6D9
- Vial size
- 150ul
Submitted references GDF11 Alleviates Pathological Myocardial Remodeling in Diabetic Cardiomyopathy Through SIRT1-Dependent Regulation of Oxidative Stress and Apoptosis.
Deficiency of Sirtuin 1 Impedes Endometrial Decidualization in Recurrent Implantation Failure Patients.
circGLI3 Inhibits Oxidative Stress by Regulating the miR-339-5p/VEGFA Axis in IPEC-J2 Cells.
ZnT8 Deficiency Protects From APAP-Induced Acute Liver Injury by Reducing Oxidative Stress Through Upregulating Hepatic Zinc and Metallothioneins.
Jian-Pi-Yi-Shen formula enhances perindopril inhibition of chronic kidney disease progression by activation of SIRT3, modulation of mitochondrial dynamics, and antioxidant effects.
Autophagy-dependent ferroptosis contributes to cisplatin-induced hearing loss.
Remodelling of gut microbiota by Berberine attenuates trimethylamine N-oxide-induced platelet hyperreaction and thrombus formation.
Silencing TAK1 reduces MAPKs-MMP2/9 expression to reduce inflammation-driven neurohistological disruption post spinal cord injury.
Effect of conditioned medium from neural stem cells on glioma progression and its protein expression profile analysis.
MEL endomorphins act as potent inflammatory analgesics with the inhibition of activated non-neuronal cells and modulation of pro-inflammatory cytokines.
Hypermethylation-mediated silencing of NDRG4 promotes pancreatic ductal adenocarcinoma by regulating mitochondrial function.
Paraoxonase 3 functions as a chaperone to decrease functional expression of the epithelial sodium channel.
Elevating ATP-binding cassette transporter G1 improves re-endothelialization function of endothelial progenitor cells via Lyn/Akt/eNOS in diabetic mice.
Different miR-21-3p isoforms and their different features in colorectal cancer.
Coordinate regulatory osteogenesis effects of icariin, timosaponin B II and ferulic acid from traditional Chinese medicine formulas on UMR-106 osteoblastic cells and osteoblasts in neonatal rat calvaria cultures.
Zhu HZ, Zhang LY, Zhai ME, Xia L, Cao Y, Xu L, Li KF, Jiang LQ, Shi H, Li X, Zhou YN, Ding W, Wang DX, Gao EH, Liu JC, Yu SQ, Duan WX
Frontiers in cell and developmental biology 2021;9:686848
Frontiers in cell and developmental biology 2021;9:686848
Deficiency of Sirtuin 1 Impedes Endometrial Decidualization in Recurrent Implantation Failure Patients.
Li J, Qi J, Yao G, Zhu Q, Li X, Xu R, Zhu Z, Zhao H, Wang Y, Ding Y, Sun Y
Frontiers in cell and developmental biology 2021;9:598364
Frontiers in cell and developmental biology 2021;9:598364
circGLI3 Inhibits Oxidative Stress by Regulating the miR-339-5p/VEGFA Axis in IPEC-J2 Cells.
Li ZX, Wang LX, Zhang Y, Chen W, Zeng YQ
BioMed research international 2021;2021:1086206
BioMed research international 2021;2021:1086206
ZnT8 Deficiency Protects From APAP-Induced Acute Liver Injury by Reducing Oxidative Stress Through Upregulating Hepatic Zinc and Metallothioneins.
Su W, Feng M, Liu Y, Cao R, Liu Y, Tang J, Pan K, Lan R, Mao Z
Frontiers in pharmacology 2021;12:721471
Frontiers in pharmacology 2021;12:721471
Jian-Pi-Yi-Shen formula enhances perindopril inhibition of chronic kidney disease progression by activation of SIRT3, modulation of mitochondrial dynamics, and antioxidant effects.
Liu X, Deng R, Wei X, Wang Y, Weng J, Lao Y, Lu J, Xiong G, Li S
Bioscience reports 2021 Oct 29;41(10)
Bioscience reports 2021 Oct 29;41(10)
Autophagy-dependent ferroptosis contributes to cisplatin-induced hearing loss.
Jian B, Pang J, Xiong H, Zhang W, Zhan T, Su Z, Lin H, Zhang H, He W, Zheng Y
Toxicology letters 2021 Oct 10;350:249-260
Toxicology letters 2021 Oct 10;350:249-260
Remodelling of gut microbiota by Berberine attenuates trimethylamine N-oxide-induced platelet hyperreaction and thrombus formation.
Xie Z, Liu X, Huang X, Liu Q, Yang M, Huang D, Zhao P, Tian J, Wang X, Hou J
European journal of pharmacology 2021 Nov 15;911:174526
European journal of pharmacology 2021 Nov 15;911:174526
Silencing TAK1 reduces MAPKs-MMP2/9 expression to reduce inflammation-driven neurohistological disruption post spinal cord injury.
Jiang S, Wu Y, Wu S, Ye S, Kong R, Chang J, Xia M, Bao J, Peng X, Hong X, Qian Z, Li H
Cell death discovery 2021 May 8;7(1):96
Cell death discovery 2021 May 8;7(1):96
Effect of conditioned medium from neural stem cells on glioma progression and its protein expression profile analysis.
Zhang GL, Qian C, Zhang SZ, Tuo YH, Zeng BY, Ji YX, Wang YZ
World journal of stem cells 2020 Nov 26;12(11):1396-1409
World journal of stem cells 2020 Nov 26;12(11):1396-1409
MEL endomorphins act as potent inflammatory analgesics with the inhibition of activated non-neuronal cells and modulation of pro-inflammatory cytokines.
Cui JM, Zhao L, Wang ZJ, Ma MT, Wang Y, Luo KY, Wang LQ, Wei S, Zhang XH, Han CZ, Liu X, Wang R
Neuropharmacology 2020 May 15;168:107992
Neuropharmacology 2020 May 15;168:107992
Hypermethylation-mediated silencing of NDRG4 promotes pancreatic ductal adenocarcinoma by regulating mitochondrial function.
Shi HH, Liu HE, Luo XJ
BMB reports 2020 Dec;53(12):658-663
BMB reports 2020 Dec;53(12):658-663
Paraoxonase 3 functions as a chaperone to decrease functional expression of the epithelial sodium channel.
Shi S, Montalbetti N, Wang X, Rush BM, Marciszyn AL, Baty CJ, Tan RJ, Carattino MD, Kleyman TR
The Journal of biological chemistry 2020 Apr 10;295(15):4950-4962
The Journal of biological chemistry 2020 Apr 10;295(15):4950-4962
Elevating ATP-binding cassette transporter G1 improves re-endothelialization function of endothelial progenitor cells via Lyn/Akt/eNOS in diabetic mice.
Shi Y, Lv X, Liu Y, Li B, Liu M, Yan M, Liu Y, Li Q, Zhang X, He S, Zhu M, He J, Zhu Y, Zhu Y, Ai D
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Dec;32(12):6525-6536
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Dec;32(12):6525-6536
Different miR-21-3p isoforms and their different features in colorectal cancer.
Jiao W, Leng X, Zhou Q, Wu Y, Sun L, Tan Y, Ni H, Dong X, Shen T, Liu Y, Li J
International journal of cancer 2017 Nov 15;141(10):2103-2111
International journal of cancer 2017 Nov 15;141(10):2103-2111
Coordinate regulatory osteogenesis effects of icariin, timosaponin B II and ferulic acid from traditional Chinese medicine formulas on UMR-106 osteoblastic cells and osteoblasts in neonatal rat calvaria cultures.
Li M, Zhang ND, Wang Y, Han T, Jiang YP, Rahman K, Qin LP, Xin HL, Zhang QY
Journal of ethnopharmacology 2016 Jun 5;185:120-31
Journal of ethnopharmacology 2016 Jun 5;185:120-31
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Western blot of Hela cell with anti-GAPDH (60004-1-Ig) at various dilutions.
- Sample type
- cell line
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The GAPDH antibody from Proteintech is a mouse monoclonal antibody to a recombinant protein of human GAPDH. This antibody recognizes human,mouse,rat antigen. The GAPDH antibody has been validated for the following applications: ELISA, IHC, WB, IP, IF analysis.
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Ethacrynic acid treated HeLa cells using 60004-1-Ig( GAPDH Antibody) at dilution of 1:50 and N/A
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP result of anti-GAPDH (60004-1-Ig for IP and Detection) with HeLa cell lysate.
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human breast cancer using 60004-1-Ig(GAPDH antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human breast cancer using 60004-1-Ig(GAPDH antibody) at dilution of 1:50 (under 40x lens)
- Sample type
- tissue
