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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- R34148 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- GAPDH Antibody
- Antibody type
- Polyclonal
- Antigen
- Amino acids GVNHEKYDNSLK were used as the immunogen for this GAPDH antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store the GAPDH antibody at -20°C.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) liver, 2) testis and 3) tonsil lysate with GAPDH antibody at 0.1/0.03/0.03 ug/ml, respectively. Predicted molecular weight ~36 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) mouse liver and 2) rat heart lysate with GAPDH antibody at 0.1 and 0.03 ug/ml, respectively. Predicted molecular weight ~36 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF/ICC testing of fixed and permeabilized human HeLa cells with GAPDH antibody (green) at 5ug/ml and DAPI nuclear stain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human liver with GAPDH antibody at 2ug/ml. HIER: steamed with pH6 citrate buffer, HRP-staining.
