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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB10014 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10014, RRID:AB_1674680
- Product name
- GAPDH polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide corresponding to N-terminus of human GAPDH.
- Reactivity
- Human, Yeast
- Host
- Rabbit
- Vial size
- 100 μg
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The macrophage cell surface glyceraldehyde-3-phosphate dehydrogenase is a novel transferrin receptor.
Interaction of TPPP/p25 protein with glyceraldehyde-3-phosphate dehydrogenase and their co-localization in Lewy bodies.
Triosephosphate isomerase- and glyceraldehyde-3-phosphate dehydrogenase-reactive autoantibodies in the cerebrospinal fluid of patients with multiple sclerosis.
Raje CI, Kumar S, Harle A, Nanda JS, Raje M
The Journal of biological chemistry 2007 Feb 2;282(5):3252-61
The Journal of biological chemistry 2007 Feb 2;282(5):3252-61
Interaction of TPPP/p25 protein with glyceraldehyde-3-phosphate dehydrogenase and their co-localization in Lewy bodies.
Oláh J, Tokési N, Vincze O, Horváth I, Lehotzky A, Erdei A, Szájli E, Medzihradszky KF, Orosz F, Kovács GG, Ovádi J
FEBS letters 2006 Oct 30;580(25):5807-14
FEBS letters 2006 Oct 30;580(25):5807-14
Triosephosphate isomerase- and glyceraldehyde-3-phosphate dehydrogenase-reactive autoantibodies in the cerebrospinal fluid of patients with multiple sclerosis.
Kolln J, Ren HM, Da RR, Zhang Y, Spillner E, Olek M, Hermanowicz N, Hilgenberg LG, Smith MA, van den Noort S, Qin Y
Journal of immunology (Baltimore, Md. : 1950) 2006 Oct 15;177(8):5652-8
Journal of immunology (Baltimore, Md. : 1950) 2006 Oct 15;177(8):5652-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using GAPDH polyclonal antibody (Cat # PAB10014) shows detection of a single band at ~39 kDa corresponding to GAPDH (arrowhead) in HeLa cell lysates.The predicted MW of GAPDH is 36 kDa.GAPDH was detected using 125 ng/ml (lane A), 250 ng/ml (lane B) and 500 ng/ml (lane C) concentrations of primary antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining using GAPDH polyclonal antibody (Cat # PAB10014) at 10 ug/mL shows detection of GAPDH in human liver tissue. Tissue was fixed in 4% paraformaldehyde using a section 4.2 um thick.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
