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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- AB0049-500 - Provider product page
- Provider
- SICGEN
- Proper citation
- SICGEN Cat#AB0049-500, RRID:AB_2333142
- Product name
- Anti-GAPDH
- Antibody type
- Polyclonal
- Description
- Goat polyclonal to GAPDH (glyceraldehyde 3-phosphate dehydrogenase). GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Porcine, Rabbit, Sheep, Simian, Other
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- 2 mg/ml
- Storage
- Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Hyperglycemia-induced degradation of HIF-1α contributes to impaired response of cardiomyocytes to hypoxia.
Inefficient recruitment of kinesin-1 to melanosomes precludes it from facilitating their transport.
K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy.
AMSH-mediated deubiquitination of Cx43 regulates internalization and degradation of gap junctions.
Arl13b and the non-muscle myosin heavy chain IIA are required for circular dorsal ruffle formation and cell migration.
Ramalho AR, Toscano A, Pereira P, Girão H, Gonçalves L, Marques C
Revista portuguesa de cardiologia : orgao oficial da Sociedade Portuguesa de Cardiologia = Portuguese journal of cardiology : an official journal of the Portuguese Society of Cardiology 2017 May;36(5):367-373
Revista portuguesa de cardiologia : orgao oficial da Sociedade Portuguesa de Cardiologia = Portuguese journal of cardiology : an official journal of the Portuguese Society of Cardiology 2017 May;36(5):367-373
Inefficient recruitment of kinesin-1 to melanosomes precludes it from facilitating their transport.
Robinson CL, Evans RD, Briggs DA, Ramalho JS, Hume AN
Journal of cell science 2017 Jun 15;130(12):2056-2065
Journal of cell science 2017 Jun 15;130(12):2056-2065
K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy.
Ferreira JV, Soares AR, Ramalho JS, Pereira P, Girao H
Scientific reports 2015 May 11;5:10210
Scientific reports 2015 May 11;5:10210
AMSH-mediated deubiquitination of Cx43 regulates internalization and degradation of gap junctions.
Ribeiro-Rodrigues TM, Catarino S, Marques C, Ferreira JV, Martins-Marques T, Pereira P, Girão H
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Nov;28(11):4629-41
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Nov;28(11):4629-41
Arl13b and the non-muscle myosin heavy chain IIA are required for circular dorsal ruffle formation and cell migration.
Casalou C, Seixas C, Portelinha A, Pintado P, Barros M, Ramalho JS, Lopes SS, Barral DC
Journal of cell science 2014 Jun 15;127(Pt 12):2709-22
Journal of cell science 2014 Jun 15;127(Pt 12):2709-22
No comments: Submit comment
Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- Cell lysates at 50 µg per lane
- Sample type
- 293A, AtT-20
- Primary Ab dilution
- 1/500
- Conjugate
- Unconjugated
- Secondary Ab
- Secondary Ab
Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- cells were fixed with methanol
- Sample type
- Hepa1-6 cell line
- Primary Ab dilution
- 1/50
- Conjugate
- Unconjugated
