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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB212-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human GDNF Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human GDNF in ELISAs and Western blots. In sandwich ELISAs, no cross-reactivity or interference was observed with recombinant human (rh) BDNF, rhCNTF, rhNeurturin, rh beta-NGF, rhNT-3, or rhNT-4.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P39905- Isotype
- IgG
- Antibody clone number
- 27106
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references PSA-NCAM-dependent GDNF signaling limits neurodegeneration and epileptogenesis in temporal lobe epilepsy.
Duveau V, Fritschy JM
The European journal of neuroscience 2010 Jul;32(1):89-98
The European journal of neuroscience 2010 Jul;32(1):89-98
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Proliferation Induced by GDNF and Neutralization by Human GDNF Antibody. Recombinant Human GDNF (Catalog # 212-GD) induces proliferation in the SH-SY5Y human neuroblastoma cell line in the presence of Recombinant Human GFR alpha-1/GDNF R alpha-1 Fc Chimera (Catalog # 714-GR) in a dose-dependent manner (orange line), as measured by the Rezazurin (Catalog # AR002). Under these conditions, proliferation elicited by GDNF (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human GDNF Monoclonal Antibody (Catalog # MAB212). The ND50 is typically 0.4-2 μg/mL.
