AF1715

antibody from Novus Biologicals

Targeting: HGFAC HGFA, HGFAP

Western blot (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1715

Provider

Novus Biologicals

Product name

Goat Polyclonal HGF Activator Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects mouse Pro-HGF Activator in direct ELISAs. In direct ELISAs, less than 4% cross-reactivity with the active form of recombinant mouse (rm) HGF Activator and no cross-reactivity with rmHGF propeptide and rmHGF is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

HGF Activator in Mouse Intestine. HGF Activator was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse Pro-HGF Activator Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1715) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.