Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [21]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [3]
- Immunohistochemistry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX113340 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113340, RRID:AB_11173017
- Product name
- Grp78 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Chicken/Avian
- Host
- Rabbit
Submitted references Artocarpin induces cell apoptosis in human osteosarcoma cells through endoplasmic reticulum stress and reactive oxygen species.
Fucoidan upregulates TLR4/CHOP-mediated caspase-3 and PARP activation to enhance cisplatin-induced cytotoxicity in human lung cancer cells.
Acute Heat Stress Changes Protein Expression in the Testes of a Broiler-Type Strain of Taiwan Country Chickens.
Whole genome expression profiling associates activation of unfolded protein response with impaired production and release of epinephrine after recurrent hypoglycemia.
HPW-RX40 prevents human platelet activation by attenuating cell surface protein disulfide isomerases.
Fucoidan induces Toll-like receptor 4-regulated reactive oxygen species and promotes endoplasmic reticulum stress-mediated apoptosis in lung cancer.
Endoplasmic reticulum-targeting photosensitizer Hypericin confers chemo-sensitization towards oxaliplatin through inducing pro-death autophagy.
Achromatopsia mutations target sequential steps of ATF6 activation.
EZH2 inhibitors transcriptionally upregulate cytotoxic autophagy and cytoprotective unfolded protein response in human colorectal cancer cells.
Host glycosylation pathways and the unfolded protein response contribute to the infection by Francisella.
Endoplasmic reticulum stress regulates inflammation and insulin resistance in skeletal muscle from pregnant women.
Endoplasmic reticulum stress is increased in adipose tissue of women with gestational diabetes.
Rosiglitazone activation of PPARγ-dependent signaling is neuroprotective in mutant huntingtin expressing cells.
The unfolded protein response is shaped by the NMD pathway.
Mutations in the unfolded protein response regulator ATF6 cause the cone dysfunction disorder achromatopsia.
Development of a multifunctional luciferase reporters system for assessing endoplasmic reticulum-targeting photosensitive compounds.
Identification of up- and down-regulated proteins in doxorubicin-resistant uterine cancer cells: reticulocalbin-1 plays a key role in the development of doxorubicin-associated resistance.
Proteomic analysis of rhein-induced cyt: ER stress mediates cell death in breast cancer cells.
High glucose-induced proteome alterations in hepatocytes and its possible relevance to diabetic liver disease.
Proteomic identification of VEGF-dependent protein enrichment to membrane caveolar-raft microdomains in endothelial progenitor cells.
Quercetin-induced cardioprotection against doxorubicin cytotoxicity.
Lee CW, Chi MC, Chang TM, Liu JF
Journal of cellular physiology 2019 Aug;234(8):13157-13168
Journal of cellular physiology 2019 Aug;234(8):13157-13168
Fucoidan upregulates TLR4/CHOP-mediated caspase-3 and PARP activation to enhance cisplatin-induced cytotoxicity in human lung cancer cells.
Hsu HY, Lin TY, Hu CH, Shu DTF, Lu MK
Cancer letters 2018 Sep 28;432:112-120
Cancer letters 2018 Sep 28;432:112-120
Acute Heat Stress Changes Protein Expression in the Testes of a Broiler-Type Strain of Taiwan Country Chickens.
Wang SH, Cheng CY, Chen CJ, Chan HL, Chen HH, Tang PC, Chen CF, Lee YP, Huang SY
Animal biotechnology 2018 Mar 19;:1-17
Animal biotechnology 2018 Mar 19;:1-17
Whole genome expression profiling associates activation of unfolded protein response with impaired production and release of epinephrine after recurrent hypoglycemia.
Kim JL, La Gamma EF, Estabrook T, Kudrick N, Nankova BB
PloS one 2017;12(2):e0172789
PloS one 2017;12(2):e0172789
HPW-RX40 prevents human platelet activation by attenuating cell surface protein disulfide isomerases.
Kung PH, Hsieh PW, Lin YT, Lee JH, Chen IH, Wu CC
Redox biology 2017 Oct;13:266-277
Redox biology 2017 Oct;13:266-277
Fucoidan induces Toll-like receptor 4-regulated reactive oxygen species and promotes endoplasmic reticulum stress-mediated apoptosis in lung cancer.
Hsu HY, Lin TY, Lu MK, Leng PJ, Tsao SM, Wu YC
Scientific reports 2017 Mar 23;7:44990
Scientific reports 2017 Mar 23;7:44990
Endoplasmic reticulum-targeting photosensitizer Hypericin confers chemo-sensitization towards oxaliplatin through inducing pro-death autophagy.
Lin S, Yang L, Shi H, Du W, Qi Y, Qiu C, Liang X, Shi W, Liu J
The international journal of biochemistry & cell biology 2017 Jun;87:54-68
The international journal of biochemistry & cell biology 2017 Jun;87:54-68
Achromatopsia mutations target sequential steps of ATF6 activation.
Chiang WC, Chan P, Wissinger B, Vincent A, Skorczyk-Werner A, Krawczyński MR, Kaufman RJ, Tsang SH, Héon E, Kohl S, Lin JH
Proceedings of the National Academy of Sciences of the United States of America 2017 Jan 10;114(2):400-405
Proceedings of the National Academy of Sciences of the United States of America 2017 Jan 10;114(2):400-405
EZH2 inhibitors transcriptionally upregulate cytotoxic autophagy and cytoprotective unfolded protein response in human colorectal cancer cells.
Hsieh YY, Lo HL, Yang PM
American journal of cancer research 2016;6(8):1661-80
American journal of cancer research 2016;6(8):1661-80
Host glycosylation pathways and the unfolded protein response contribute to the infection by Francisella.
Barel M, Harduin-Lepers A, Portier L, Slomianny MC, Charbit A
Cellular microbiology 2016 Dec;18(12):1763-1781
Cellular microbiology 2016 Dec;18(12):1763-1781
Endoplasmic reticulum stress regulates inflammation and insulin resistance in skeletal muscle from pregnant women.
Liong S, Lappas M
Molecular and cellular endocrinology 2016 Apr 15;425:11-25
Molecular and cellular endocrinology 2016 Apr 15;425:11-25
Endoplasmic reticulum stress is increased in adipose tissue of women with gestational diabetes.
Liong S, Lappas M
PloS one 2015;10(4):e0122633
PloS one 2015;10(4):e0122633
Rosiglitazone activation of PPARγ-dependent signaling is neuroprotective in mutant huntingtin expressing cells.
Chiang MC, Cheng YC, Nicol CJ, Lin KH, Yen CH, Chen SJ, Huang RN
Experimental cell research 2015 Nov 1;338(2):183-93
Experimental cell research 2015 Nov 1;338(2):183-93
The unfolded protein response is shaped by the NMD pathway.
Karam R, Lou CH, Kroeger H, Huang L, Lin JH, Wilkinson MF
EMBO reports 2015 May;16(5):599-609
EMBO reports 2015 May;16(5):599-609
Mutations in the unfolded protein response regulator ATF6 cause the cone dysfunction disorder achromatopsia.
Kohl S, Zobor D, Chiang WC, Weisschuh N, Staller J, Gonzalez Menendez I, Chang S, Beck SC, Garcia Garrido M, Sothilingam V, Seeliger MW, Stanzial F, Benedicenti F, Inzana F, Héon E, Vincent A, Beis J, Strom TM, Rudolph G, Roosing S, Hollander AI, Cremers FP, Lopez I, Ren H, Moore AT, Webster AR, Michaelides M, Koenekoop RK, Zrenner E, Kaufman RJ, Tsang SH, Wissinger B, Lin JH
Nature genetics 2015 Jul;47(7):757-65
Nature genetics 2015 Jul;47(7):757-65
Development of a multifunctional luciferase reporters system for assessing endoplasmic reticulum-targeting photosensitive compounds.
Lin S, Zhang L, Lei K, Zhang A, Liu P, Liu J
Cell stress & chaperones 2014 Nov;19(6):927-37
Cell stress & chaperones 2014 Nov;19(6):927-37
Identification of up- and down-regulated proteins in doxorubicin-resistant uterine cancer cells: reticulocalbin-1 plays a key role in the development of doxorubicin-associated resistance.
May EW, Lin ST, Lin CC, Chang JF, Hung E, Lo YW, Lin LH, Hu RY, Feng CL, Lin DY, Wu SB, Lee WC, Lyu KW, Chou HC, Chan HL
Pharmacological research 2014 Dec;90:1-17
Pharmacological research 2014 Dec;90:1-17
Proteomic analysis of rhein-induced cyt: ER stress mediates cell death in breast cancer cells.
Huang HJ, Lin CC, Chou HC, Chen YW, Lin ST, Lin YC, Lin DY, Lyu KW, Chan HL
Molecular bioSystems 2014 Dec;10(12):3086-100
Molecular bioSystems 2014 Dec;10(12):3086-100
High glucose-induced proteome alterations in hepatocytes and its possible relevance to diabetic liver disease.
Chen JY, Chou HC, Chen YH, Chan HL
The Journal of nutritional biochemistry 2013 Nov;24(11):1889-910
The Journal of nutritional biochemistry 2013 Nov;24(11):1889-910
Proteomic identification of VEGF-dependent protein enrichment to membrane caveolar-raft microdomains in endothelial progenitor cells.
Chillà A, Magherini F, Margheri F, Laurenzana A, Gamberi T, Bini L, Bianchi L, Danza G, Mazzanti B, Serratì S, Modesti A, Del Rosso M, Fibbi G
Molecular & cellular proteomics : MCP 2013 Jul;12(7):1926-38
Molecular & cellular proteomics : MCP 2013 Jul;12(7):1926-38
Quercetin-induced cardioprotection against doxorubicin cytotoxicity.
Chen JY, Hu RY, Chou HC
Journal of biomedical science 2013 Dec 20;20:95
Journal of biomedical science 2013 Dec 20;20:95
No comments: Submit comment
Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HepG2 whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody (GTX113340) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7.5% SDS PAGE GTX113340 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Grp78 antibody detects Grp78 protein by western blot analysis.A. 30 £gg 293T whole cell lysate/extractB. 30 £gg A431 whole cell lysate/extract C. 30 £gg HeLa whole cell lysate/extractD. 30 £gg HepG2 whole cell lysate/extract7.5 % SDS-PAGEGrp78 antibody (GTX113340) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Grp78 antibody detects Grp78 protein by western blot analysis.A. 30 ?g PC-12 whole cell extract B. 30 ?g Rat2 whole cell extract7.5% SDS-PAGEGrp78 antibody (GTX113340) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody (GTX113340) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HepG2 whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody (GTX113340) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Grp78 antibody detects Grp78 protein at cytoplasm by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Grp78 protein stained by Grp78 antibody (GTX113340) diluted at 1:500.Blue: Hoechst 33342 staining.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Grp78 antibody detects Grp78 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Grp78 protein stained by Grp78 antibody (GTX113340) diluted at 1:500.Blue: Hoechst 33342 staining.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Grp78 antibody detects Grp78 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Grp78 protein stained by Grp78 antibody (GTX113340) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using Grp78(GTX113340) antibody at 1:250 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Grp78 antibody detects Grp78 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Grp78 antibody (GTX113340) diluted at 1:250.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Grp78 antibody detects Grp78 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Grp78 antibody (GTX113340) diluted at 1:250.
