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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- ARG54027 - Provider product page
- Provider
- Arigo
- Product name
- anti-BiP / GRP78 antibody
- Antibody type
- Monoclonal
- Antigen
- Purified recombinant human BiP/GRP78 (C-terminus) protein fragments expressed in E.coli.
- Description
- Affinity purified
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 9E4-2A7-H6
- Vial size
- 100 µl
- Concentration
- 0.8 mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: BiP/GRP78 (C-terminus) in HeLa, C6, Lncap and MDA-MB-468 cell lysates stained with BiP / GRP78 antibody [9E4-2A7-H6]inal (ARG54027) (diluted in 1: 1000). Predicted band size: 72KDa. Observed band size: 78KDa.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: HeLa cells fixed with 4% Paraformaldehyde and stained with BiP / GRP78 antibody [9E4-2A7-H6], C-terminal (ARG54027) (diluted in 1:50).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded Colorectal cancer stained with BiP / GRP78 antibody [9E4-2A7-H6], C-terminal (ARG54027) (diluted in 1/100). Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6. 0).
