NBP1-06274

antibody from Novus Biologicals

Targeting: HSPA5 BiP, GRP78

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-06274

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-06274, RRID:AB_1555284

Product name

Rabbit Polyclonal GRP78/HSPA5 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat, Chicken/Avian, Drosophila, Sheep

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

1.0 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Auto-regulation of Secretory Flux by Sensing and Responding to the Folded Cargo Protein Load in the Endoplasmic Reticulum.

Subramanian A, Capalbo A, Iyengar NR, Rizzo R, di Campli A, Di Martino R, Lo Monte M, Beccari AR, Yerudkar A, Del Vecchio C, Glielmo L, Turacchio G, Pirozzi M, Kim SG, Henklein P, Cancino J, Parashuraman S, Diviani D, Fanelli F, Sallese M, Luini A
Cell 2019 Mar 7;176(6):1461-1476.e23

---

Age- and stress-associated C. elegans granulins impair lysosomal function and induce a compensatory HLH-30/TFEB transcriptional response.

Butler VJ, Gao F, Corrales CI, Cortopassi WA, Caballero B, Vohra M, Ashrafi K, Cuervo AM, Jacobson MP, Coppola G, Kao AW
PLoS genetics 2019 Aug;15(8):e1008295

---

Binge ethanol exposure induces endoplasmic reticulum stress in the brain of adult mice.

Wang Y, Wang X, Li H, Xu M, Frank J, Luo J
Toxicology and applied pharmacology 2018 Oct 1;356:172-181

---

Ethanol-induced damage to the developing spinal cord: The involvement of CCR2 signaling.

Ren Z, Wang X, Yang F, Xu M, Frank JA, Wang H, Wang S, Ke ZJ, Luo J
Biochimica et biophysica acta. Molecular basis of disease 2017 Nov;1863(11):2746-2761

---

Arrestins contribute to amyloid beta-induced cell death via modulation of autophagy and the α7nAch receptor in SH-SY5Y cells.

Liu YQ, Jia MQ, Xie ZH, Liu XF, Hui-Yang, Zheng XL, Yuan HQ, Bi JZ
Scientific reports 2017 Jun 13;7(1):3446

---

Bitter melon protects against ER stress in LS174T colonic epithelial cells.

Kunde DA, Chong WC, Nerurkar PV, Ahuja KD, Just J, Smith JA, Guven N, Eri RD
BMC complementary and alternative medicine 2017 Jan 3;17(1):2

---

Endoplasmic Reticulum Stress and Autophagy in Homocystinuria Patients with Remethylation Defects.

Martínez-Pizarro A, Desviat LR, Ugarte M, Pérez B, Richard E
PloS one 2016;11(3):e0150357

---

Increased Glucose Transport into Neurons Rescues Aβ Toxicity in Drosophila.

Niccoli T, Cabecinha M, Tillmann A, Kerr F, Wong CT, Cardenes D, Vincent AJ, Bettedi L, Li L, Grönke S, Dols J, Partridge L
Current biology : CB 2016 Sep 12;26(17):2291-300

---

A GRP78-Directed Monoclonal Antibody Recaptures Response in Refractory Multiple Myeloma with Extramedullary Involvement.

Rasche L, Menoret E, Dubljevic V, Menu E, Vanderkerken K, Lapa C, Steinbrunn T, Chatterjee M, Knop S, Düll J, Greenwood DL, Hensel F, Rosenwald A, Einsele H, Brändlein S
Clinical cancer research : an official journal of the American Association for Cancer Research 2016 Sep 1;22(17):4341-9

---

Chronic plus binge ethanol exposure causes more severe pancreatic injury and inflammation.

Ren Z, Yang F, Wang X, Wang Y, Xu M, Frank JA, Ke ZJ, Zhang Z, Shi X, Luo J
Toxicology and applied pharmacology 2016 Oct 1;308:11-19

---

Ablation of Perk in Schwann Cells Improves Myelination in the S63del Charcot-Marie-Tooth 1B Mouse.

Sidoli M, Musner N, Silvestri N, Ungaro D, D'Antonio M, Cavener DR, Feltri ML, Wrabetz L
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Nov 2;36(44):11350-11361

---

Binge ethanol exposure causes endoplasmic reticulum stress, oxidative stress and tissue injury in the pancreas.

Ren Z, Wang X, Xu M, Yang F, Frank JA, Ke ZJ, Luo J
Oncotarget 2016 Aug 23;7(34):54303-54316

---

Cell type-specific transcriptomics of hypothalamic energy-sensing neuron responses to weight-loss.

Henry FE, Sugino K, Tozer A, Branco T, Sternson SM
eLife 2015 Sep 2;4

---

Acyl-CoA synthetase 1 deficiency alters cardiolipin species and impairs mitochondrial function.

Grevengoed TJ, Martin SA, Katunga L, Cooper DE, Anderson EJ, Murphy RC, Coleman RA
Journal of lipid research 2015 Aug;56(8):1572-82

---

Caspase-12 mediates carbon tetrachloride-induced hepatocyte apoptosis in mice.

Liu H, Wang Z, Nowicki MJ
World journal of gastroenterology 2014 Dec 28;20(48):18189-98

---

Paneth cells as a site of origin for intestinal inflammation.

Adolph TE, Tomczak MF, Niederreiter L, Ko HJ, Böck J, Martinez-Naves E, Glickman JN, Tschurtschenthaler M, Hartwig J, Hosomi S, Flak MB, Cusick JL, Kohno K, Iwawaki T, Billmann-Born S, Raine T, Bharti R, Lucius R, Kweon MN, Marciniak SJ, Choi A, Hagen SJ, Schreiber S, Rosenstiel P, Kaser A, Blumberg RS
Nature 2013 Nov 14;503(7475):272-6

---

NOX2 deficiency attenuates markers of adiposopathy and brain injury induced by high-fat diet.

Pepping JK, Freeman LR, Gupta S, Keller JN, Bruce-Keller AJ
American journal of physiology. Endocrinology and metabolism 2013 Feb 15;304(4):E392-404

---

Cell surface relocalization of the endoplasmic reticulum chaperone and unfolded protein response regulator GRP78/BiP.

Zhang Y, Liu R, Ni M, Gill P, Lee AS
The Journal of biological chemistry 2010 May 14;285(20):15065-75

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: GRP78/HSPA5 Antibody [NBP1-06274] - Image shows a specific band for GRP78 in 0.1 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GRP78/HSPA5 Antibody [NBP1-06274] - Detection of Bip/Grp78 on HeLa whole cell extracts using NBP1-06274.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GRP78/HSPA5 Antibody [NBP1-06274] - Total protein from human HeLa and A431 cells, mouse 3T3 cells and rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-GPR78 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: GRP78/HSPA5 Antibody [NBP1-06274] - Analysis using Alexa Fluor (R) 488 conjugate of NBP1-06274. An intracellular stain was performed on HeLa cells with GPR78/HSPA5 antibody NBP1-06274 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. 1 ug of antibody was added to 100 uL of staining buffer and cells were incubated for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: GRP78/HSPA5 Antibody [NBP1-06274] - An intracellular stain was performed on Jurkat cells with Alexa Fluor 647 conjugate of GPR78/HSPA5 antibody NBP1-06274AF647 (blue) and a matched isotype control NBP2-24893AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: GRP78/HSPA5 Antibody [NBP1-06274] - An intracellular stain was performed on HeLa with NBP1-06274 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: GRP78/HSPA5 Antibody [NBP1-06274] - An intracellular stain was performed on NIH3T3 cells with GRP78/HSPA5 Antibody NBP1-06274AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.