Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- F54005 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- GRP78 Antibody / BiP
- Antibody type
- Monoclonal
- Description
- Purified
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 4F11-D5-C10
- Vial size
- 0.1 ml
- Storage
- Store the GRP78 antibody at -20°C.
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of mouse NIH3T3, human A549, rat C6 and human HeLa cell lysates using GRP78 antibody at 1:1000. Predicted molecular weight: ~73kDa, but routinely observed at 70-78kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC/IF testing of HeLa cells, fixed by anhydrous methanol at -20oC, with GRP78 antibody at 1:200.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunoprecipitation from HeLa cell lysate using GRP78 antibody. Blot was subsequently probed with same mAb.
