LS-C774263

antibody from LSBio

Targeting: HSPA5 BiP, GRP78

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Enhanced validation)

Antibody Data

Product number

LS-C774263

Provider

LSBio

Product name

HSPA5 / GRP78 / BiP Antibody (clone 3C5-1A4, FITC) LS-C774263

Antibody type

Monoclonal

Description

Protein G purified

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Green dye

Isotype

IgG

Antibody clone number

3C5-1A4

Storage

Store at -20°C.

Immunocytochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4. Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4. Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.