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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- F54266 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- GRP78 Antibody / BiP / HSPA5
- Antibody type
- Polyclonal
- Description
- SAS precipitation
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.05 µl, 0.2 µl
- Storage
- Aliquot the HSPA5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human HL60 cell lysate with HSPA5 antibody. Predicted molecular weight: ~73 kDa, but routinely observed at 70-78 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of mouse liver cell lysate with HSPA5 antibody. Predicted molecular weight: ~73 kDa, but routinely observed at 70-78 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of fixed and permeabilized human NCI-H460 cells with HSPA5 antibody (green) and DAPI nuclear stain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human testis tissue with HSPA5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human prostate carcinoma with HSPA5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human HeLa cells with HSPA5 antibody; Blue=isotype control, Green= HSPA5 antibody.
