11760-1-AP

antibody from Invitrogen Antibodies

Targeting: HNRNPC HNRPC

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11760-1-AP

Provider

Invitrogen Antibodies

Product name

HNRNPC Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MASNVTNKT DPRSMNSRVF IGNLNTLVVK KSDVEAIFSK YGKIVGCSVH KGFAFVQYVN ERNARAAVAG EDGRMIAGQV LDINLAAEPK VNRGKAGVKR SAAEMYGSSF DLDYDFQRDY YDRMYSYPAR VPPPPPIARA VVPSKRQRVS GNTSRRGKSG FNSKSGQRGS SKSGKLKGDD LQAIKKELTQ IKQKVDSLLE NLEKIEKEQS KQAVEMKNDK SEEEQSSSSV KKDETNVKME SEGGADDSAE EGDLLDDDDN EDRGDDQLEL IKDDEKEAEE GEDDRDSANG EDDS (1-293 aa encoded by BC003394)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of HNRNPC antibody (11760-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNRNPC transfected HeLa cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 11760-1-AP (HNRNPC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 11760-1-AP (HNRNPC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using HNRNPC antibody 11760-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lymphoma using 11760-1-AP (HNRNPC antibody) at dilution of 1:100 (under 10x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HeLa cells were stained with 0.2ug HNRNPC antibody (11760-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-HNRNPC (IP:11760-1-AP, 4ug; Detection:11760-1-AP 1:500) with HeLa cells lysate 1080ug.