H00002157-M03

antibody from Abnova Corporation

Targeting: F8 DXS1253E, F8C, FVIII, HEMA

ELISA (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002157-M03

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002157-M03, RRID:AB_606210

Product name

F8 monoclonal antibody (M03), clone 1E8

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant F8.

Antigen sequence

KFILLFAVFDEGKSWHSETKNSLMQDRDAASARAW
PKMHTVNGYVNRSLPGLIGCHRKSVYWHVIGMGTT
PEVHSIFLEGHTFLVRNHRQASLEISPITF


Isotype

IgG

Antibody clone number

1E8

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged F8 is approximately 3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between CALR and F8. HeLa cells were stained with anti-CALR rabbit purified polyclonal 1:1200 and anti-F8 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)