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LearnMA5-34662
antibody from Invitrogen Antibodies
Targeting: SET
2PP2A, IGAAD, IPP2A2, PHAPII, TAF-I, TAF-IBETA
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [5]
- Other assay [1]
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- Product number
- MA5-34662 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SET Recombinant Rabbit Monoclonal Antibody (JB60-34)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: K562, Hela, LOVO, MCF-7, human tonsil tissue, human kidney tissue, human lung cancer tissue, mouse fallopian tube tissue, rat kidney tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JB60-34
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Bi-Functional Peptides as a New Therapeutic Tool for Hepatocellular Carcinoma.
Savier E, Simon-Gracia L, Charlotte F, Tuffery P, Teesalu T, Scatton O, Rebollo A
Pharmaceutics 2021 Oct 6;13(10)
Pharmaceutics 2021 Oct 6;13(10)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SET in K562 cell lysates. Samples were incubated with SET monoclonal antibody (Product # MA5-34662), at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SET in MCF-7 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with SET monoclonal antibody (Product # MA5-34662), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SET in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with SET monoclonal antibody (Product # MA5-34662), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SET in HeLa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with SET monoclonal antibody (Product # MA5-34662), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SET in paraffin-embedded rat kidney tissue. Samples were incubated with SET monoclonal antibody (Product # MA5-34662), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SET in paraffin-embedded mouse fallopian tube tissue. Samples were incubated with SET monoclonal antibody (Product # MA5-34662), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SET in paraffin-embedded human tonsil tissue. Samples were incubated with SET monoclonal antibody (Product # MA5-34662), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SET in paraffin-embedded human lung cancer tissue. Samples were incubated with SET monoclonal antibody (Product # MA5-34662), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SET in paraffin-embedded human kidney tissue. Samples were incubated with SET monoclonal antibody (Product # MA5-34662), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 The IP disrupted PP2A/SET interaction in vitro. Lysates were immunoprecipitated with anti PP2A antibody. The PP2A/SET interaction was competed in vitro with 1 mM of the PP2A/SET IP and with an irrelevant Ras/Raf IP used as a negative control. The total amount of PP2A was used as internal control.
