21738-1-AP

antibody from Invitrogen Antibodies

Targeting: ITGB4 CD104

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

21738-1-AP

Provider

Invitrogen Antibodies

Product name

Integrin beta-4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: ANRCKKAPV KSCTECVRVD KDCAYCTDEM FRDRRCNTQA ELLAAGCQRE SIVVMESSFQ ITEETQIDTT LRRSQMSPQG LRVRLRPGEE RHFELEVFEP LESPVDLYIL MDFSNSMSDD LDNLKKMGQN LARVLSQLTS DYTIGFGKFV DKVSVPQTDM RPEKLKEPWP NSDPPFSFKN VISLTEDVDE FRNKLQGERI SGNLDAPEGG FDAILQTAVC TRDIGWRPDS THLLVFSTES AFHYEADGAN VLAGIMSRND ERCHLDTTGT YTQYRTQDYP SVPTLVRLLA KHNIIPIFAV TNYSYSYYEK LHTYFPVSSL GVLQEDSSN (27-354 aa encoded by BC126411)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.26 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF7 cells were subjected to SDS PAGE followed by western blot with 21738-1-AP (ITGB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A431 cells were subjected to SDS PAGE followed by western blot with 21738-1-AP (ITGB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 21738-1-AP (ITGB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas cancer using 21738-1-AP (ITGB4 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas cancer using 21738-1-AP (ITGB4 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 21738-1-AP (ITGB4 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 21738-1-AP (ITGB4 antibody) at dilution of 1:50 (under 40x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HeLa cells were stained with 0.2ug Integrin beta-4 antibody (21738-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-Integrin beta-4 (IP:21738-1-AP, 4ug; Detection:21738-1-AP 1:500) with HeLa cells lysate 2400ug.