- NBP2-22217 - Novus Biologicals LAMP2 antibody

Submitted references Ubiquitylation of the ER-Shaping Protein Lunapark via the CRL3^KLHL12 Ubiquitin Ligase Complex.

Yuniati L, Lauriola A, Gerritsen M, Abreu S, Ni E, Tesoriero C, Onireti JO, Low TY, Heck AJR, Vettori A, Cardozo T, Guardavaccaro D
Cell reports 2020 May 19;31(7):107664

Human serum albumin-based probes for molecular targeting of macrophage scavenger receptors.

Ahmed M, Baumgartner R, Aldi S, Dusart P, Hedin U, Gustafsson B, Caidahl K
International journal of nanomedicine 2019;14:3723-3741

Intestinal epithelial tight junction barrier regulation by autophagy-related protein ATG6/beclin 1.

Wong M, Ganapathy AS, Suchanec E, Laidler L, Ma T, Nighot P
American journal of physiology. Cell physiology 2019 May 1;316(5):C753-C765

Mitophagy inhibits amyloid-β and tau pathology and reverses cognitive deficits in models of Alzheimer's disease.

Fang EF, Hou Y, Palikaras K, Adriaanse BA, Kerr JS, Yang B, Lautrup S, Hasan-Olive MM, Caponio D, Dan X, Rocktäschel P, Croteau DL, Akbari M, Greig NH, Fladby T, Nilsen H, Cader MZ, Mattson MP, Tavernarakis N, Bohr VA
Nature neuroscience 2019 Mar;22(3):401-412

Iron Supply via NCOA4-Mediated Ferritin Degradation Maintains Mitochondrial Functions.

Fujimaki M, Furuya N, Saiki S, Amo T, Imamichi Y, Hattori N
Molecular and cellular biology 2019 Jul 15;39(14)

Chick Embryo: A Preclinical Model for Understanding Ischemia-Reperfusion Mechanism.

Fauzia E, Barbhuyan TK, Shrivastava AK, Kumar M, Garg P, Khan MA, Robertson AAB, Raza SS
Frontiers in pharmacology 2018;9:1034

Mutations in GFAP Disrupt the Distribution and Function of Organelles in Human Astrocytes.

Jones JR, Kong L, Hanna MG 4th, Hoffman B, Krencik R, Bradley R, Hagemann T, Choi J, Doers M, Dubovis M, Sherafat MA, Bhattacharyya A, Kendziorski C, Audhya A, Messing A, Zhang SC
Cell reports 2018 Oct 23;25(4):947-958.e4

Histone deacetylase 6 controls Notch3 trafficking and degradation in T-cell acute lymphoblastic leukemia cells.

Pinazza M, Ghisi M, Minuzzo S, Agnusdei V, Fossati G, Ciminale V, Pezzè L, Ciribilli Y, Pilotto G, Venturoli C, Amadori A, Indraccolo S
Oncogene 2018 Jul;37(28):3839-3851

Small-Vessel Vasculopathy Due to Aberrant Autophagy in LAMP-2 Deficiency.

Nguyen HT, Noguchi S, Sugie K, Matsuo Y, Nguyen CTH, Koito H, Shiojima I, Nishino I, Tsukaguchi H
Scientific reports 2018 Feb 20;8(1):3326

NDP52 interacts with mitochondrial RNA poly(A) polymerase to promote mitophagy.

Furuya N, Kakuta S, Sumiyoshi K, Ando M, Nonaka R, Suzuki A, Kazuno S, Saiki S, Hattori N
EMBO reports 2018 Dec;19(12)

LT-IIc, A Bacterial Type II Heat-Labile Enterotoxin, Induces Specific Lethality in Triple Negative Breast Cancer Cells by Modulation of Autophagy and Induction of Apoptosis and Necroptosis.

Masso-Welch P, Girald Berlingeri S, King-Lyons ND, Mandell L, Hu J, Greene CJ, Federowicz M, Cao P, Connell TD, Heakal Y
International journal of molecular sciences 2018 Dec 26;20(1)

The endothelial cell receptor stabilin-2 regulates VWF-FVIII complex half-life and immunogenicity.

Swystun LL, Lai JD, Notley C, Georgescu I, Paine AS, Mewburn J, Nesbitt K, Schledzewski K, Géraud C, Kzhyshkowska J, Goerdt S, Hopman W, Montgomery RR, James PD, Lillicrap D
The Journal of clinical investigation 2018 Aug 31;128(9):4057-4073

Mouse Tmem135 mutation reveals a mechanism involving mitochondrial dynamics that leads to age-dependent retinal pathologies.

Lee WH, Higuchi H, Ikeda S, Macke EL, Takimoto T, Pattnaik BR, Liu C, Chu LF, Siepka SM, Krentz KJ, Rubinstein CD, Kalejta RF, Thomson JA, Mullins RF, Takahashi JS, Pinto LH, Ikeda A
eLife 2016 Nov 15;5

Autophagy enhances intestinal epithelial tight junction barrier function by targeting claudin-2 protein degradation.

Nighot PK, Hu CA, Ma TY
The Journal of biological chemistry 2015 Mar 13;290(11):7234-46

Uptake and degradation of protease-sensitive and -resistant forms of abnormal human prion protein aggregates by human astrocytes.

Choi YP, Head MW, Ironside JW, Priola SA
The American journal of pathology 2014 Dec;184(12):3299-307

Arrestin-2 interacts with the ubiquitin-protein isopeptide ligase atrophin-interacting protein 4 and mediates endosomal sorting of the chemokine receptor CXCR4.

Bhandari D, Trejo J, Benovic JL, Marchese A
The Journal of biological chemistry 2007 Dec 21;282(51):36971-9

Recognition and ubiquitination of Salmonella type III effector SopA by a ubiquitin E3 ligase, HsRMA1.

Zhang Y, Higashide W, Dai S, Sherman DM, Zhou D
The Journal of biological chemistry 2005 Nov 18;280(46):38682-8

HDAC6 and microtubules are required for autophagic degradation of aggregated huntingtin.

Iwata A, Riley BE, Johnston JA, Kopito RR
The Journal of biological chemistry 2005 Dec 2;280(48):40282-92

Influences of the lysosomal associated membrane proteins (Lamp-1, Lamp-2) and Mac-2 binding protein (Mac-2-BP) on the prognosis of pancreatic carcinoma.

Künzli BM, Berberat PO, Zhu ZW, Martignoni M, Kleeff J, Tempia-Caliera AA, Fukuda M, Zimmermann A, Friess H, Büchler MW
Cancer 2002 Jan 1;94(1):228-39

Primary LAMP-2 deficiency causes X-linked vacuolar cardiomyopathy and myopathy (Danon disease).

Nishino I, Fu J, Tanji K, Yamada T, Shimojo S, Koori T, Mora M, Riggs JE, Oh SJ, Koga Y, Sue CM, Yamamoto A, Murakami N, Shanske S, Byrne E, Bonilla E, Nonaka I, DiMauro S, Hirano M
Nature 2000 Aug 24;406(6798):906-10

Distinct saturable pathways for the endocytosis of different tyrosine motifs.

Warren RA, Green FA, Stenberg PE, Enns CA
The Journal of biological chemistry 1998 Jul 3;273(27):17056-63

Distinct saturable pathways for the endocytosis of different tyrosine motifs.

Warren RA, Green FA, Stenberg PE, Enns CA
The Journal of biological chemistry 1998 Jul 3;273(27):17056-63

Release of lipopolysaccharide from intracellular compartments containing Salmonella typhimurium to vesicles of the host epithelial cell.

Garcia-del Portillo F, Stein MA, Finlay BB
Infection and immunity 1997 Jan;65(1):24-34

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Simple Western: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Lane view shows a specific band for LAMP-2/CD107b in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Analysis of LAMP-2/CD107b expression in Whole Cell Lysates: 2) HeLa, 3) Jurkat, 4) U937, 5) HepG2, Tissue Extracts: 6) Human kidney, 7) Human lung, 8) Human liver, and 9) Human placenta.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Localization of TMEM135 to the mitochondria. The mitochondrial fraction isolated from the WT mouse brain show TMEM135 signals by immunoblotting. Following proteins were used as organelle markers: MFN2-mitochondria; LAMP2-lysosome; Lamin B1- nucleus; PDI- endoplasmic reticulum (ER). Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/19264), licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Representative pictures of effect of I/R on DNA damage, cell survival, and inflammation. Western blot analysis expression level of Cathepsin B (*P < 0.05) in total protein extract from RVA of I/R treated vs. control group. Image collected and cropped by CiteAb from the following publication (https://www.frontiersin.org/article/10.3389/fphar.2018.01034/full), licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: ELISA: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Anti-LAMP-2/CD107b was used to assess lysates from human SVG-A cells via ELISA, by loading the indicated mass of total protein per well. Antibodies tested were diluted 1:250, and used in conjunction with an HRP-conjugated secondary antibody. Signal was detected by luminescence. Image from verified customer review.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Staining of LAMP-2/CD107b in human liver using DAB with hematoxylin counterstain.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using anti-LAMP-2/CD107b (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Analysis using the FITC conjugate of anti-LAMP-2/CD107b. Staining of 5 ul overlay with isotype on U937 Cells.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Using the Alexa Fluor 647 direct conjugate, an intracellular stain was performed on HeLa cells with LAMP-2/CD107b (H4B4) NBP2-22217AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - An intracellular stain was performed on SK-MEL-28 cells with LAMP-2/CD107b (H4B4) NBP2-22217AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - An intracellular stain was performed on U937 cells with HGF LAMP-2/CD107b [H4B4] Antibody NBP2-22217AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - An intracellular stain was performed on Jurkat cells with LAMP-2/CD107b [H4B4] Antibody NBP2-22217AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

NBP2-22217

Antibody data