Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [2]
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Validation data
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- Product number
- 50-1078-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD107b (LAMP-2) Monoclonal Antibody (eBioH4B4 (H4B4)), eFluor™ 660, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBioH4B4 monoclonal antibody reacts with human CD107b, also known as lysosomal-associated membrane protein-2 (LAMP-2). CD107b is a highly glycosylated, type I transmembrane protein of approximately 105 kDa. It is expressed intracellularly in lysosomal/endosomal membranes in nearly all cells. It is also expressed on the surface of degranulating T cells (to a lesser extent than CD107a) and activated platelets as well as some cancer cells. In humans, mutations in CD107b results in a lysosomal glycogen storage disorder, known as Danon disease.
- Antibody clone number
- eBioH4B4 (H4B4)
- Concentration
- 5 µL/Test
Submitted references Improved Autophagic Flux in Escapers from Doxorubicin-Induced Senescence/Polyploidy of Breast Cancer Cells.
Multifunctional Natural Killer Cell Engagers Targeting NKp46 Trigger Protective Tumor Immunity.
Lysosomal membrane permeabilization is involved in oxidative stress-induced apoptotic cell death in LAMP2-deficient iPSCs-derived cerebral cortical neurons.
Detection of T-cell degranulation: CD107a and b.
LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells.
Primary LAMP-2 deficiency causes X-linked vacuolar cardiomyopathy and myopathy (Danon disease).
Bojko A, Staniak K, Czarnecka-Herok J, Sunderland P, Dudkowska M, Śliwińska MA, Salmina K, Sikora E
International journal of molecular sciences 2020 Aug 24;21(17)
International journal of molecular sciences 2020 Aug 24;21(17)
Multifunctional Natural Killer Cell Engagers Targeting NKp46 Trigger Protective Tumor Immunity.
Gauthier L, Morel A, Anceriz N, Rossi B, Blanchard-Alvarez A, Grondin G, Trichard S, Cesari C, Sapet M, Bosco F, Rispaud-Blanc H, Guillot F, Cornen S, Roussel A, Amigues B, Habif G, Caraguel F, Arrufat S, Remark R, Romagné F, Morel Y, Narni-Mancinelli E, Vivier E
Cell 2019 Jun 13;177(7):1701-1713.e16
Cell 2019 Jun 13;177(7):1701-1713.e16
Lysosomal membrane permeabilization is involved in oxidative stress-induced apoptotic cell death in LAMP2-deficient iPSCs-derived cerebral cortical neurons.
Law CY, Siu CW, Fan K, Lai WH, Au KW, Lau YM, Wong LY, Ho JCY, Lee YK, Tse HF, Ng KM
Biochemistry and biophysics reports 2016 Mar;5:335-345
Biochemistry and biophysics reports 2016 Mar;5:335-345
Detection of T-cell degranulation: CD107a and b.
Betts MR, Koup RA
Methods in cell biology 2004;75:497-512
Methods in cell biology 2004;75:497-512
LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells.
Grützkau A, Smorodchenko A, Lippert U, Kirchhof L, Artuc M, Henz BM
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2004 Sep;61(1):62-8
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2004 Sep;61(1):62-8
Primary LAMP-2 deficiency causes X-linked vacuolar cardiomyopathy and myopathy (Danon disease).
Nishino I, Fu J, Tanji K, Yamada T, Shimojo S, Koori T, Mora M, Riggs JE, Oh SJ, Koga Y, Sue CM, Yamamoto A, Murakami N, Shanske S, Byrne E, Bonilla E, Nonaka I, DiMauro S, Hirano M
Nature 2000 Aug 24;406(6798):906-10
Nature 2000 Aug 24;406(6798):906-10
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Intracellular staining of Jurkat cells with Mouse IgG1 K Isotype Control eFluor® 660 (Product # 50-4714-82) (blue histogram) or Anti-Human CD107b (LAMP-2) eFluor® 660 (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor® 450, were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Autophagic flux resumption in descendants of polyploid/senescent MDA-MB-231 and MCF-7 cells. Cells were treated with 100 nM doxorubicin for one day, then cultured in a fresh medium and analyzed on subsequent days. ( a , c ) Representative western blots showing autophagy protein levels of p-ULK1 (S757), ULK1, LC3B, LAMP-2 and SQSTM1/p62 in MDA-MB-231 cells ( a ) and MCF-7 cells ( c ). ( b , d ) Quantitative analysis of autophagic index based on LC3B protein levels in untreated and bafilomycin A- or chloroquine-treated MDA-MB-231 cells ( b ) and MCF-7 cells ( d ) with representative western blots showing LC3B protein levels. Bars: mean value, error bars: SEM, n = 4. Statistical significance (in relation to control): $ p < 0.051, * p < 0.05, ** p < 0.01. ( e ) Transient accumulation of autophagic vesicles. TEM images show typical MDA-MB-231cells on the subsequent days following treatment (upper panel) and their magnified parts with autophagic vesicles (red arrows) and lipofuscin particles (red asterisks).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 LAMP2-deficient neurons showed increase levels of cytosolic cathepsin L. (A) Western blot analysis revealed the elevated level of active cathepsin L in the LAMP2-deficient iPSCs-derived cortical neurons under non-stressed condition. (B) Western blot analysis showed that oxidative stress increased the abundance of active cathepsin L in the cytosols. N =3; *: P