- GTX100637 - GeneTex MET antibody

Chen ST, Kuo TC, Liao YY, Lin MC, Tien YW, Huang MC
Oncogene 2018 Nov;37(46):6041-6053

Pei YF, Yin XM, Liu XQ
Biochimica et biophysica acta. Molecular basis of disease 2018 Jan;1864(1):197-207

Wang C, Tseng T, Jhang Y, Tseng J, Hsieh C, Wu WG, Lee S
Experimental dermatology 2014 Nov;23(11):843-9

Wu YM, Liu CH, Huang MJ, Lai HS, Lee PH, Hu RH, Huang MC
Cancer research 2013 Sep 1;73(17):5580-90

Lin WC, Tsai HF, Kuo SH, Wu MS, Lin CW, Hsu PI, Cheng AL, Hsu PN
Cancer research 2010 Jul 15;70(14):5740-8

No comments: Submit comment

Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [C3], C-term (GTX100637) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
KD Reagent Type: siRNA
Downregulation: >50%
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
KD Reagent Prod no: Proprietary.
KD Reagent Prod page: KD Reagent Prod page
Antibody Lot Number: 39988

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample(30 ?g whole cell lysate)A:HeLa S3(GTX14654)5% SDS PAGEGTX100637 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Wild-type (WT) and c-Met knockout (KO) HeLa cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [C3], C-term (GTX100637) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [C3], C-term (GTX100637) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Wild-type (WT) and c-Met knockout (KO) HeLa cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [C3], C-term (GTX100637) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: c-Met antibody [c3], c-term detects c-Met protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: c-Met protein stained by c-Met antibody [c3], c-term (GTX100637) diluted at 1:1000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunoprecipitation of c-Met protein from HeLa whole cell extracts using 5 £gg of c-Met antibody [C3], C-term (GTX100637).Western blot analysis was performed using c-Met antibody [C3], C-term (GTX100637).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX100637