MAB1143

antibody from Abnova Corporation

Targeting: MET DFNB97, HGFR, RCCP2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB1143

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB1143, RRID:AB_1577214

Product name

MET monoclonal antibody, clone 4AT44

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against partial recombinant MET.

Isotype

IgG

Antibody clone number

4AT44

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Increased HGF and c-Met in muscle tissues of polymyositis and dermatomyositis patients: beneficial roles of HGF in muscle regeneration.

Sugiura T, Kawaguchi Y, Soejima M, Katsumata Y, Gono T, Baba S, Kawamoto M, Murakawa Y, Yamanaka H, Hara M
Clinical immunology (Orlando, Fla.) 2010 Sep;136(3):387-99

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Further evidence for the role of MET in autism susceptibility.

Thanseem I, Nakamura K, Miyachi T, Toyota T, Yamada S, Tsujii M, Tsuchiya KJ, Anitha A, Iwayama Y, Yamada K, Hattori E, Matsuzaki H, Matsumoto K, Iwata Y, Suzuki K, Suda S, Kawai M, Sugihara G, Takebayashi K, Takei N, Ichikawa H, Sugiyama T, Yoshikawa T, Mori N
Neuroscience research 2010 Oct;68(2):137-41

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MET receptor sequence variants R970C and T992I lack transforming capacity.

Tyner JW, Fletcher LB, Wang EQ, Yang WF, Rutenberg-Schoenberg ML, Beadling C, Mori M, Heinrich MC, Deininger MW, Druker BJ, Loriaux MM
Cancer research 2010 Aug 1;70(15):6233-7

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection of endogenous MET in HepG2 cell line. 10 ug/lane of HepG2 cell lysate was used to examine the expression of human MTE. Lanes 1-5 represent MET monoclonal antibody, clone 4AT44 (Cat # MAB1143). Lane 6 represents auto-phosohorylated-MET in HepG2 cell line detected by anti-phospho-MET monoclonal antibody (Cat # MAB0115).

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human colon carcinomareacted with MET monoclonal antibody, clone 4AT44 (Cat # MAB1143), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)