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- Antibody Data
- Antigen structure
- References [17]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- AF276 - Provider product page
- Provider
- R&D Systems
- Product name
- Human HGFR/c-MET Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human HGF R/c-MET in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant mouse HGF R is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P08581- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Broad-spectrum receptor tyrosine kinase inhibitors overcome de novo and acquired modes of resistance to EGFR-targeted therapies in colorectal cancer.
The association of semaphorin 5A with lymph node metastasis and adverse prognosis in cervical cancer.
Mutant p53s generate pro-invasive niches by influencing exosome podocalyxin levels.
Tumor and Plasma Met Levels in Non-Metastatic Prostate Cancer.
Beta 1-integrin-c-Met cooperation reveals an inside-in survival signalling on autophagy-related endomembranes.
Targeting matriptase in breast cancer abrogates tumour progression via impairment of stromal-epithelial growth factor signalling.
A pharmacodynamic/pharmacokinetic study of ficlatuzumab in patients with advanced solid tumors and liver metastases.
Internalization of Met requires the co-receptor CD44v6 and its link to ERM proteins.
Mutant p53 enhances MET trafficking and signalling to drive cell scattering and invasion.
Identification of a pivotal endocytosis motif in c-Met and selective modulation of HGF-dependent aggressiveness of cancer using the 16-mer endocytic peptide.
c-Met-induced epithelial carcinogenesis is initiated by the serine protease matriptase.
Epidermal growth factor receptor regulates MET levels and invasiveness through hypoxia-inducible factor-1alpha in non-small cell lung cancer cells.
Dorsal ruffle microdomains potentiate Met receptor tyrosine kinase signaling and down-regulation.
Decorin is a novel antagonistic ligand of the Met receptor.
Effect of human patient plasma ex vivo treatment on gene expression and progenitor cell activation of primary human liver cells in multi-compartment 3D perfusion bioreactors for extra-corporeal liver support.
Impairment of the antifibrotic effect of hepatocyte growth factor in lung fibroblasts from African Americans: possible role in systemic sclerosis.
Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor.
Graves-Deal R, Bogatcheva G, Rehman S, Lu Y, Higginbotham JN, Singh B
Oncotarget 2019 Feb 12;10(13):1320-1333
Oncotarget 2019 Feb 12;10(13):1320-1333
The association of semaphorin 5A with lymph node metastasis and adverse prognosis in cervical cancer.
Xiao JB, Li XL, Liu L, Wang G, Hao SN, Dong HJ, Wang XM, Zhang YF, Liu HD
Cancer cell international 2018;18:87
Cancer cell international 2018;18:87
Mutant p53s generate pro-invasive niches by influencing exosome podocalyxin levels.
Novo D, Heath N, Mitchell L, Caligiuri G, MacFarlane A, Reijmer D, Charlton L, Knight J, Calka M, McGhee E, Dornier E, Sumpton D, Mason S, Echard A, Klinkert K, Secklehner J, Kruiswijk F, Vousden K, Macpherson IR, Blyth K, Bailey P, Yin H, Carlin LM, Morton J, Zanivan S, Norman JC
Nature communications 2018 Nov 29;9(1):5069
Nature communications 2018 Nov 29;9(1):5069
Tumor and Plasma Met Levels in Non-Metastatic Prostate Cancer.
Kaye DR, Pinto PA, Cecchi F, Reilly J, Semerjian A, Rabe DC, Gupta G, Choyke PL, Bottaro DP
PloS one 2016;11(6):e0157130
PloS one 2016;11(6):e0157130
Beta 1-integrin-c-Met cooperation reveals an inside-in survival signalling on autophagy-related endomembranes.
Barrow-McGee R, Kishi N, Joffre C, Ménard L, Hervieu A, Bakhouche BA, Noval AJ, Mai A, Guzmán C, Robbez-Masson L, Iturrioz X, Hulit J, Brennan CH, Hart IR, Parker PJ, Ivaska J, Kermorgant S
Nature communications 2016 Jun 23;7:11942
Nature communications 2016 Jun 23;7:11942
Targeting matriptase in breast cancer abrogates tumour progression via impairment of stromal-epithelial growth factor signalling.
Zoratti GL, Tanabe LM, Varela FA, Murray AS, Bergum C, Colombo É, Lang JE, Molinolo AA, Leduc R, Marsault E, Boerner J, List K
Nature communications 2015 Apr 15;6:6776
Nature communications 2015 Apr 15;6:6776
A pharmacodynamic/pharmacokinetic study of ficlatuzumab in patients with advanced solid tumors and liver metastases.
Tabernero J, Elez ME, Herranz M, Rico I, Prudkin L, Andreu J, Mateos J, Carreras MJ, Han M, Gifford J, Credi M, Yin W, Agarwal S, Komarnitsky P, Baselga J
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 May 15;20(10):2793-804
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 May 15;20(10):2793-804
Internalization of Met requires the co-receptor CD44v6 and its link to ERM proteins.
Hasenauer S, Malinger D, Koschut D, Pace G, Matzke A, von Au A, Orian-Rousseau V
PloS one 2013;8(4):e62357
PloS one 2013;8(4):e62357
Mutant p53 enhances MET trafficking and signalling to drive cell scattering and invasion.
Muller PA, Trinidad AG, Timpson P, Morton JP, Zanivan S, van den Berghe PV, Nixon C, Karim SA, Caswell PT, Noll JE, Coffill CR, Lane DP, Sansom OJ, Neilsen PM, Norman JC, Vousden KH
Oncogene 2013 Mar 7;32(10):1252-65
Oncogene 2013 Mar 7;32(10):1252-65
Identification of a pivotal endocytosis motif in c-Met and selective modulation of HGF-dependent aggressiveness of cancer using the 16-mer endocytic peptide.
Cho KW, Park JH, Park CW, Lee D, Lee E, Kim DJ, Kim KJ, Yoon SH, Park Y, Kim E, Cho S, Jang S, Park BC, Chi SW, Yoo SH, Jang MH, Kim HN, Kim E, Jo K, Park YW
Oncogene 2013 Feb 21;32(8):1018-29
Oncogene 2013 Feb 21;32(8):1018-29
c-Met-induced epithelial carcinogenesis is initiated by the serine protease matriptase.
Szabo R, Rasmussen AL, Moyer AB, Kosa P, Schafer JM, Molinolo AA, Gutkind JS, Bugge TH
Oncogene 2011 Apr 28;30(17):2003-16
Oncogene 2011 Apr 28;30(17):2003-16
Epidermal growth factor receptor regulates MET levels and invasiveness through hypoxia-inducible factor-1alpha in non-small cell lung cancer cells.
Xu L, Nilsson MB, Saintigny P, Cascone T, Herynk MH, Du Z, Nikolinakos PG, Yang Y, Prudkin L, Liu D, Lee JJ, Johnson FM, Wong KK, Girard L, Gazdar AF, Minna JD, Kurie JM, Wistuba II, Heymach JV
Oncogene 2010 May 6;29(18):2616-27
Oncogene 2010 May 6;29(18):2616-27
Dorsal ruffle microdomains potentiate Met receptor tyrosine kinase signaling and down-regulation.
Abella JV, Parachoniak CA, Sangwan V, Park M
The Journal of biological chemistry 2010 Aug 6;285(32):24956-67
The Journal of biological chemistry 2010 Aug 6;285(32):24956-67
Decorin is a novel antagonistic ligand of the Met receptor.
Goldoni S, Humphries A, Nyström A, Sattar S, Owens RT, McQuillan DJ, Ireton K, Iozzo RV
The Journal of cell biology 2009 May 18;185(4):743-54
The Journal of cell biology 2009 May 18;185(4):743-54
Effect of human patient plasma ex vivo treatment on gene expression and progenitor cell activation of primary human liver cells in multi-compartment 3D perfusion bioreactors for extra-corporeal liver support.
Schmelzer E, Mutig K, Schrade P, Bachmann S, Gerlach JC, Zeilinger K
Biotechnology and bioengineering 2009 Jul 1;103(4):817-27
Biotechnology and bioengineering 2009 Jul 1;103(4):817-27
Impairment of the antifibrotic effect of hepatocyte growth factor in lung fibroblasts from African Americans: possible role in systemic sclerosis.
Bogatkevich GS, Ludwicka-Bradley A, Highland KB, Hant F, Nietert PJ, Singleton CB, Feghali-Bostwick CA, Silver RM
Arthritis and rheumatism 2007 Jul;56(7):2432-42
Arthritis and rheumatism 2007 Jul;56(7):2432-42
Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor.
Skibinski G, Elborn JS, Ennis M
American journal of physiology. Lung cellular and molecular physiology 2007 Jul;293(1):L69-76
American journal of physiology. Lung cellular and molecular physiology 2007 Jul;293(1):L69-76
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Western Blot Shows Human HGF R/c-MET Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HGF R/c-Met knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HGF R/c-MET Antigen Affinity-purified Polyclonal Antibody (Catalog # AF276) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for HGF R/c-MET at approximately 150-200 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- HGF R/c-MET in HT-29 and U937 Human Cell Line. HGF R/c-MET was detected in immersion fixed HT-29 human colon adenocarcinoma cell line (positive control, left panel) and U937 human histiocytic lymphoma cell line (negative control, right panel) using Goat Anti-Human HGF R/c-MET Antigen Affinity-purified Polyclonal Antibody (Catalog # AF276) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- HGF R/c-MET in Human Liver. HGF R/c-MET was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human HGF R/c-MET Antigen Affinity-purified Polyclonal Antibody (Catalog # AF276) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- HGF R/c-MET in Human Skin. HGF R/c-MET was detected in immersion fixed paraffin-embedded sections of human skin using 15 µg/mL Goat Anti-Human HGF R/c-MET Antigen Affinity-purified Polyclonal Antibody (Catalog # AF276) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
