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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP52648PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP52648PU-N, RRID:AB_11143311
- Product name
- anti MeCP2 (N-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide selected from the N-terminal region of Human MeCP2.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- 0.5 mg/mL
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of MeCP2 Antibody (N-term) Cat.-No AP52648PU-N in MDA-MB435 cell line lysates (35ug/lane). This demonstrates the MeCP2 antibody detected the MeCP2 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin fixed, paraffin embedded human brain tissue stained with MeCP2 Antibody (N-term) Cat.-No AP52648PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MDA-MB435 cells using MeCP2 Antibody (N-term) Cat.-No AP52648PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
