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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- STJA0003679 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-MECP2 antibody [5H12] (STJA0003679)
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody anti-MeCP2 is suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry research applications.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NA- Epitope
- NA
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20°C, and avoid repeated freeze-thaw cycles.
- Handling
- NA
No comments: Submit comment
Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunofluorescence of a section of rat cerebellum showing specific labeling of MeCP2 (cat. STJA0003679, 1:1000, red) in nuclei of neurons and specific labeling of GFAP ( cat. STJA0003659, 1:5000, green) in the network of astroglial cells and projections of Bergmann glia, and Hoechst staining of nuclear DNA.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunofluorescence of a section of rat cerebellum labeled with anti-MeCP2 (cat. 1205-MECP2, 1:500, red) , colabeled with anti-GFAP ( cat. STJA0003658, 1:5000, green) , and DAPI staining of nuclear DNA. The anti-GFAP labels the astrocytic cells and the processes of Bergmann glia in the molecular layer.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunostaining of a section of rat hippocampus showing specific nuclear immunolabeling of MeCP2 (cat. 1205-MECP2, red, 1:1000) and FOX3 (green). The blue is DAPI staining of nuclear DNA.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
