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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- MA5-14282 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDKN2C Monoclonal Antibody (18P118 (DCS-118))
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-14282 targets p18INK4c in IF, IHC (P), IP, and WB applications and shows reactivity with Human and mouse samples.
- Antibody clone number
- 18P118 (DCS-118)
- Concentration
- 0.4 mg/mL
Submitted references An opposite effect of the CDK inhibitor, p18(INK4c) on embryonic stem cells compared with tumor and adult stem cells.
Identification of prognostic biomarkers for glioblastomas using protein expression profiling.
Upregulation of p18Ink4c expression by oncogenic HPV E6 via p53-miR-34a pathway.
The cyclin-dependent kinase inhibitors p15INK4B and p21CIP1 are critical regulators of fibrillar collagen-induced tumor cell cycle arrest.
Proliferation and differentiation in isogenic populations of peripheral B cells activated by Epstein-Barr virus or T cell-derived mitogens.
Proliferation and differentiation in isogenic populations of peripheral B cells activated by Epstein-Barr virus or T cell-derived mitogens.
Li Y, Pal R, Sung LY, Feng H, Miao W, Cheng SY, Tian C, Cheng T
PloS one 2012;7(9):e45212
PloS one 2012;7(9):e45212
Identification of prognostic biomarkers for glioblastomas using protein expression profiling.
Jung Y, Joo KM, Seong DH, Choi YL, Kong DS, Kim Y, Kim MH, Jin J, Suh YL, Seol HJ, Shin CS, Lee JI, Kim JH, Song SY, Nam DH
International journal of oncology 2012 Apr;40(4):1122-32
International journal of oncology 2012 Apr;40(4):1122-32
Upregulation of p18Ink4c expression by oncogenic HPV E6 via p53-miR-34a pathway.
Wang X, Meyers C, Guo M, Zheng ZM
International journal of cancer 2011 Sep 15;129(6):1362-72
International journal of cancer 2011 Sep 15;129(6):1362-72
The cyclin-dependent kinase inhibitors p15INK4B and p21CIP1 are critical regulators of fibrillar collagen-induced tumor cell cycle arrest.
Wall SJ, Zhong ZD, DeClerck YA
The Journal of biological chemistry 2007 Aug 17;282(33):24471-6
The Journal of biological chemistry 2007 Aug 17;282(33):24471-6
Proliferation and differentiation in isogenic populations of peripheral B cells activated by Epstein-Barr virus or T cell-derived mitogens.
O'Nions J, Allday MJ
The Journal of general virology 2004 Apr;85(Pt 4):881-95
The Journal of general virology 2004 Apr;85(Pt 4):881-95
Proliferation and differentiation in isogenic populations of peripheral B cells activated by Epstein-Barr virus or T cell-derived mitogens.
O'Nions J, Allday MJ
The Journal of general virology 2004 Apr;85(Pt 4):881-895
The Journal of general virology 2004 Apr;85(Pt 4):881-895
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of p18INK4c using p18INK4c Monoclonal Antibody (Product # MA5-14282) on HeLa Cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of CDKN2C was achieved by transfecting HeLa cells with CDKN2C specific siRNAs (Silencer® select Product # s2847). Western blot analysis (Fig a) was performed using Whole cell extract from the CDKN2C knock down cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Anti-CDKN2C Mouse Monoclonal Antibody (Product # MA5-14282, 1 µg/mL) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to CDKN2C.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of Raji (Lane 1), Ramos (Lane 2), SK-OV-3 (Lane 3) and HeLa (Lane 4). The blots were probed with Mouse anti-CDKN2C Monoclonal Antibody (Product # MA5-14282, 1 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). An 18 kDa band corresponding to CDKN2C was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human tonsil stained with p18 antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of p18INK4c using p18INK4c Monoclonal Antibody (Product # MA5-14282) on Native Human LS174T Cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of p18INK4c using p18INK4c Monoclonal Antibody (Product # MA5-14282) on Native Human LS174T Cells.
