sc-311

antibody from Santa Cruz Biotechnology

Targeting: RAB1A RAB1, YPT1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-311

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-311, RRID:AB_632290

Product name

Anti-RAB1A

Antibody type

Polyclonal (Antigen purified)

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

References

Submitted references

Alpha-synuclein delays endoplasmic reticulum (ER)-to-Golgi transport in mammalian cells by antagonizing ER/Golgi SNAREs.

Thayanidhi N, Helm JR, Nycz DC, Bentley M, Liang Y, Hay JC
Molecular biology of the cell 2010 Jun 1;21(11):1850-63

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Cell type-specific and light-dependent expression of Rab1 and Rab6 GTPases in mammalian retinas.

Huang W, Wu G, Wang GY
Visual neuroscience 2009 Nov;26(5-6):443-52

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Author Index for Abstracts of the American Society of Nephrology Meeting

Kidney International 1985 Jan;27(1):353-374

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Single band corresponding to the predicted size in kDa (+/-20%).

Primary Ab dilution

1:500

Secondary Ab dilution

1:3000

Lane 1

Marker [kDa]: 220, 112, 84, 47, 32, 26, 16.8

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 22

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.