NBP1-55113

antibody from Novus Biologicals

Targeting: RAB1A RAB1, YPT1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-55113

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-55113, RRID:AB_11023755

Product name

Rabbit Polyclonal Rab1A Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Simian

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Storage

Store at -20C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Rab1A Antibody [NBP1-55113] - HeLa, Vera, HeLa transfected with mouse construct, Antibody Titration: 0.2-1 ug/ml

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Rab1A Antibody [NBP1-55113] - 1. Human Cervical Cancer Cell lysate (15ug) 2. Monkey Fibroblast Cell lysate (15ug) 3. Human Cervical Cancer Cell transfected with Rab1A-GFP (15ug) Primary Dilution: 1 : 1000 Secondary Antibody: goat anti-Rabbit Secondary Dilution: 1 : 40,000 .

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Rab1A Antibody [NBP1-55113] - Sample Tissue: HepG2, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 2ug/ml, Peptide Concentration: 5.0 ug/ml, Lysate Quantity: 25ug/lane/lane, Gel Concentration: 12%

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Rab1A Antibody [NBP1-55113] - 293T Whole Cell lysates, Antibody Dilution: 0.2 ug/ml.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Rab1A Antibody [NBP1-55113] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:312500 Positive Control: Human Muscle

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Rab1A Antibody [NBP1-55113] - Analysis of HepG2 cell lysate. Antibody Dilution: 1.0 ug/ml.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Rab1A Antibody [NBP1-55113] - Formalin Fixed Paraffin Embedded Human Bronchial Epithelial Tissue. Observed Staining: Cytoplasmic staining with Primary Antibody Concentration: 1:100 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec.