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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00008738-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00008738-M01, RRID:AB_547566
- Product name
- Mouse Monoclonal RAIDD/CRADD Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CRADD - CASP2 and RIPK1 domain containing adaptor with death domain
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Mutations in CRADD Result in Reduced Caspase-2-Mediated Neuronal Apoptosis and Cause Megalencephaly with a Rare Lissencephaly Variant.
Di Donato N, Jean YY, Maga AM, Krewson BD, Shupp AB, Avrutsky MI, Roy A, Collins S, Olds C, Willert RA, Czaja AM, Johnson R, Stover JA, Gottlieb S, Bartholdi D, Rauch A, Goldstein A, Boyd-Kyle V, Aldinger KA, Mirzaa GM, Nissen A, Brigatti KW, Puffenberger EG, Millen KJ, Strauss KA, Dobyns WB, Troy CM, Jinks RN
American journal of human genetics 2016 Nov 3;99(5):1117-1129
American journal of human genetics 2016 Nov 3;99(5):1117-1129
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RAIDD/CRADD Antibody (1F8) [H00008738-M01] - Analysis of CRADD expression in transfected 293T cell line by CRADD monoclonal antibody (M01), clone 1F8.Lane 1: CRADD transfected lysate(23 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RAIDD/CRADD Antibody (1F8) [H00008738-M01] - CRADD monoclonal antibody (M01), clone 1F8 Analysis of CRADD expression in K-562.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: RAIDD/CRADD Antibody (1F8) [H00008738-M01] - Detection limit for recombinant GST tagged CRADD is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: RAIDD/CRADD Antibody (1F8) [H00008738-M01] - Analysis of monoclonal antibody to CRADD on HeLa cell. Antibody concentration 10 ug/ml.
