LS-C817929

antibody from LSBio

Targeting: CRADD RAIDD

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

LS-C817929

Provider

LSBio

Product name

CRADD / RAIDD Antibody (Preservative Free) LS-C817929

Antibody type

Polyclonal

Description

Protein A affinity chromatography

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

CRADD was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate. 1 uL anti-CRADD rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-CRADD rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 23 kDa.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

CRADD was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate. 1 uL anti-CRADD rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-CRADD rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 23 kDa.