PA5-80501

antibody from Invitrogen Antibodies

Targeting: CRADD RAIDD

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-80501

Provider

Invitrogen Antibodies

Product name

RAIDD Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human CRADD/RAIDD.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RAIDD in Lane A: K562 Whole Cell Lysate (30 µg). Samples were probed using a RAIDD Polyclonal Antibody (Product # PA5-80501) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:23 kDa. Observed band size:23 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using RAIDD Polyclonal Antibody (Product # PA5-80501) at 1:500 dilution. Lane A: K562 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti- RabbitIgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 23 kDa. (We are unsure of the identity of these extra bands).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of RAIDD in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with RAIDD Polyclonal Antibody (Product # PA5-80501, 1:5,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human RAIDD in human kidney with RAIDD Polyclonal Antibody (Product # PA5-80501, 1:5,000, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human RAIDD in human liver with RAIDD Polyclonal Antibody (Product # PA5-80501, 1:5,000, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RAIDD Immunoprecipitation using: Lane A: 0.5 mg K562 Whole Cell Lysate, Lane B: 0.5 mg Hela Whole Cell Lysate 1 µL with RAIDD Polyclonal Antibody (Product # PA5-80501) and 15 µL of 50 % Protein G agarose. Primary antibody: RAIDD Polyclonal Antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 23 kDa.