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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- sc-1247 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-1247, RRID:AB_2302969
- Product name
- Anti-TAF9
- Antibody type
- Polyclonal (Antigen purified)
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Goat
Submitted references And-1 is required for the stability of histone acetyltransferase Gcn5
STAGA recruits Mediator to the MYC oncoprotein to stimulate transcription and cell proliferation.
Y Li, A N Jaramillo-Lambert, Y Yang, R Williams, N H Lee, W Zhu
Oncogene 2011 Jul;():-
Oncogene 2011 Jul;():-
STAGA recruits Mediator to the MYC oncoprotein to stimulate transcription and cell proliferation.
Liu X, Vorontchikhina M, Wang YL, Faiola F, Martinez E
Molecular and cellular biology 2008 Jan;28(1):108-21
Molecular and cellular biology 2008 Jan;28(1):108-21
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:2000
- Lane 1
- Marker [kDa]: 220, 112, 84, 47, 32, 26, 16.8
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 29
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human small intestine shows strong nuclear positivity in glandular cells.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
