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Learnsc-280
antibody from Santa Cruz Biotechnology
Targeting: PTPN11
BPTP3, NS1, PTP2C, SH-PTP2, SHP-2, SHP2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [15]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-280 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-280, RRID:AB_632401
- Product name
- Anti-PTPN11
- Antibody type
- Polyclonal (Antigen purified)
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
Submitted references PTP1B and SHP2 in POMC neurons reciprocally regulate energy balance in mice.
Temporal requirement of the protein tyrosine phosphatase Shp2 in establishing the neuronal fate in early retinal development.
Sprouty2-modulated Kras signaling rescues Shp2 deficiency during lens and lacrimal gland development.
Down-regulation of protein-tyrosine phosphatases activates an immune receptor in the absence of its translocation into lipid rafts.
Dopamine regulates phosphorylation of VEGF receptor 2 by engaging Src-homology-2-domain-containing protein tyrosine phosphatase 2.
Shp2 acts downstream of SDF-1alpha/CXCR4 in guiding granule cell migration during cerebellar development.
Identification of c-Src tyrosine kinase substrates in platelet-derived growth factor receptor signaling.
Signal strength dictates phosphoinositide 3-kinase contribution to Ras/extracellular signal-regulated kinase 1 and 2 activation via differential Gab1/Shp2 recruitment: consequences for resistance to epidermal growth factor receptor inhibition.
Phosphatase SHP-1 promotes TLR- and RIG-I-activated production of type I interferon by inhibiting the kinase IRAK1
Distinct requirements for Gab1 in Met and EGF receptor signaling in vivo.
Clonal population structure and specific genotypes of multidrug-resistant Campylobacter coli from Turkeys.
Additive effects of PDGF receptor beta signaling pathways in vascular smooth muscle cell development.
Identification of YB-1 as a regulator of PTP1B expression: implications for regulation of insulin and cytokine signaling.
Expression of Gab1 lacking the pleckstrin homology domain is associated with neoplastic progression.
Erythroid cells rendered erythropoietin independent by infection with Friend spleen focus-forming virus show constitutive activation of phosphatidylinositol 3-kinase and Akt kinase: involvement of insulin receptor substrate-related adapter proteins.
Banno R, Zimmer D, De Jonghe BC, Atienza M, Rak K, Yang W, Bence KK
The Journal of clinical investigation 2010 Mar;120(3):720-34
The Journal of clinical investigation 2010 Mar;120(3):720-34
Temporal requirement of the protein tyrosine phosphatase Shp2 in establishing the neuronal fate in early retinal development.
Cai Z, Feng GS, Zhang X
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010 Mar 17;30(11):4110-9
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010 Mar 17;30(11):4110-9
Sprouty2-modulated Kras signaling rescues Shp2 deficiency during lens and lacrimal gland development.
Pan Y, Carbe C, Powers A, Feng GS, Zhang X
Development (Cambridge, England) 2010 Apr;137(7):1085-93
Development (Cambridge, England) 2010 Apr;137(7):1085-93
Down-regulation of protein-tyrosine phosphatases activates an immune receptor in the absence of its translocation into lipid rafts.
Heneberg P, Dráberová L, Bambousková M, Pompach P, Dráber P
The Journal of biological chemistry 2010 Apr 23;285(17):12787-802
The Journal of biological chemistry 2010 Apr 23;285(17):12787-802
Dopamine regulates phosphorylation of VEGF receptor 2 by engaging Src-homology-2-domain-containing protein tyrosine phosphatase 2.
Sinha S, Vohra PK, Bhattacharya R, Dutta S, Sinha S, Mukhopadhyay D
Journal of cell science 2009 Sep 15;122(Pt 18):3385-92
Journal of cell science 2009 Sep 15;122(Pt 18):3385-92
Shp2 acts downstream of SDF-1alpha/CXCR4 in guiding granule cell migration during cerebellar development.
Hagihara K, Zhang EE, Ke YH, Liu G, Liu JJ, Rao Y, Feng GS
Developmental biology 2009 Oct 1;334(1):276-84
Developmental biology 2009 Oct 1;334(1):276-84
Identification of c-Src tyrosine kinase substrates in platelet-derived growth factor receptor signaling.
Amanchy R, Zhong J, Hong R, Kim JH, Gucek M, Cole RN, Molina H, Pandey A
Molecular oncology 2009 Dec;3(5-6):439-50
Molecular oncology 2009 Dec;3(5-6):439-50
Signal strength dictates phosphoinositide 3-kinase contribution to Ras/extracellular signal-regulated kinase 1 and 2 activation via differential Gab1/Shp2 recruitment: consequences for resistance to epidermal growth factor receptor inhibition.
Sampaio C, Dance M, Montagner A, Edouard T, Malet N, Perret B, Yart A, Salles JP, Raynal P
Molecular and cellular biology 2008 Jan;28(2):587-600
Molecular and cellular biology 2008 Jan;28(2):587-600
Phosphatase SHP-1 promotes TLR- and RIG-I-activated production of type I interferon by inhibiting the kinase IRAK1
Huazhang An, Jin Hou, Jun Zhou, Wei Zhao, Hongmei Xu, Yuejuan Zheng, Yizhi Yu, Shuxun Liu, Xuetao Cao
Nature Immunology 2008 Apr;9(5):542-550
Nature Immunology 2008 Apr;9(5):542-550
Distinct requirements for Gab1 in Met and EGF receptor signaling in vivo.
Schaeper U, Vogel R, Chmielowiec J, Huelsken J, Rosario M, Birchmeier W
Proceedings of the National Academy of Sciences of the United States of America 2007 Sep 25;104(39):15376-81
Proceedings of the National Academy of Sciences of the United States of America 2007 Sep 25;104(39):15376-81
Clonal population structure and specific genotypes of multidrug-resistant Campylobacter coli from Turkeys.
D'lima CB, Miller WG, Mandrell RE, Wright SL, Siletzky RM, Carver DK, Kathariou S
Applied and environmental microbiology 2007 Apr;73(7):2156-64
Applied and environmental microbiology 2007 Apr;73(7):2156-64
Additive effects of PDGF receptor beta signaling pathways in vascular smooth muscle cell development.
Tallquist MD, French WJ, Soriano P
PLoS biology 2003 Nov;1(2):E52
PLoS biology 2003 Nov;1(2):E52
Identification of YB-1 as a regulator of PTP1B expression: implications for regulation of insulin and cytokine signaling.
Fukada T, Tonks NK
The EMBO journal 2003 Feb 3;22(3):479-93
The EMBO journal 2003 Feb 3;22(3):479-93
Expression of Gab1 lacking the pleckstrin homology domain is associated with neoplastic progression.
Kameda H, Risinger JI, Han BB, Baek SJ, Barrett JC, Abe T, Takeuchi T, Glasgow WC, Eling TE
Molecular and cellular biology 2001 Oct;21(20):6895-905
Molecular and cellular biology 2001 Oct;21(20):6895-905
Erythroid cells rendered erythropoietin independent by infection with Friend spleen focus-forming virus show constitutive activation of phosphatidylinositol 3-kinase and Akt kinase: involvement of insulin receptor substrate-related adapter proteins.
Nishigaki K, Hanson C, Ohashi T, Thompson D, Muszynski K, Ruscetti S
Journal of virology 2000 Apr;74(7):3037-45
Journal of virology 2000 Apr;74(7):3037-45
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 220, 112, 84, 47, 32, 26, 16.8
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 68
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
- Validation comment
- Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.
