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Learn20145-1-AP
antibody from Proteintech Group
Targeting: PTPN11
BPTP3, NS1, PTP2C, SH-PTP2, SHP-2, SHP2
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [21]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- 20145-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#20145-1-AP, RRID:AB_10699877
- Product name
- SHP2 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated SHP2 antibody (Cat. #20145-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Inhibition of Phosphodiesterase 5 Promotes the Aromatase-Mediated Estrogen Biosynthesis in Osteoblastic Cells by Activation of cGMP/PKG/SHP2 Pathway.
Brevilin A Inhibits STAT3 Signaling and Induces ROS-Dependent Apoptosis, Mitochondrial Stress and Endoplasmic Reticulum Stress in MCF-7 Breast Cancer Cells.
Inhibition of JNK-Mediated Autophagy Promotes Proscillaridin A- Induced Apoptosis via ROS Generation, Intracellular Ca+2 Oscillation and Inhibiting STAT3 Signaling in Breast Cancer Cells.
Shp2 activation in bone marrow microenvironment mediates the drug resistance of B-cell acute lymphoblastic leukemia through enhancing the role of VCAM-1/VLA-4.
miR-552 Regulates Liver Tumor-Initiating Cell Expansion and Sorafenib Resistance.
The role of tyrosine phosphatase Shp2 in spermatogonial differentiation and spermatocyte meiosis.
Anticancer activity of dietary xanthone α-mangostin against hepatocellular carcinoma by inhibition of STAT3 signaling via stabilization of SHP1.
Glycochenodeoxycholic acid induces stemness and chemoresistance via the STAT3 signaling pathway in hepatocellular carcinoma cells.
A thiazole-derived oridonin analogue exhibits antitumor activity by directly and allosterically inhibiting STAT3.
Overexpression of miR-489 derails mammary hierarchy structure and inhibits HER2/neu-induced tumorigenesis.
Proscillaridin A Promotes Oxidative Stress and ER Stress, Inhibits STAT3 Activation, and Induces Apoptosis in A549 Lung Adenocarcinoma Cells.
Proscillaridin A induces apoptosis, inhibits STAT3 activation and augments doxorubicin toxicity in prostate cancer cells.
Chemical and genetic inhibition of STAT3 sensitizes hepatocellular carcinoma cells to sorafenib induced cell death.
The abnormal activation of D1R/Shp-2 complex involved in levodopa-induced dyskinesia in 6-hydroxydopamine-lesioned Parkinson's rats.
Levodopa/Benserazide Loaded Microspheres Alleviate L-dopa Induced Dyskinesia through Preventing the Over-Expression of D1R/Shp-2/ERK1/2 Signaling Pathway in a Rat Model of Parkinson's Disease.
Shp2 promotes liver cancer stem cell expansion by augmenting β-catenin signaling and predicts chemotherapeutic response of patients.
Cryptotanshinone inhibits human glioma cell proliferation in vitro and in vivo through SHP-2-dependent inhibition of STAT3 activation.
Dietary quercetin potentiates the antiproliferative effect of interferon-α in hepatocellular carcinoma cells through activation of JAK/STAT pathway signaling by inhibition of SHP2 phosphatase.
Expression and clinical significance of tyrosine phosphatase SHP2 in thyroid carcinoma.
Chemical composition of total flavonoids from Polygonum amplexicaule and their pro-apoptotic effect on hepatocellular carcinoma cells: Potential roles of suppressing STAT3 signaling.
Systemic lupus erythematosus immune complexes increase the expression of SLAM family members CD319 (CRACC) and CD229 (LY-9) on plasmacytoid dendritic cells and CD319 on CD56(dim) NK cells.
Wisanwattana W, Wongkrajang K, Cao DY, Shi XK, Zhang ZH, Zhou ZY, Li F, Mei QG, Wang C, Suksamrarn A, Zhang GL, Wang F
Frontiers in endocrinology 2021;12:636784
Frontiers in endocrinology 2021;12:636784
Brevilin A Inhibits STAT3 Signaling and Induces ROS-Dependent Apoptosis, Mitochondrial Stress and Endoplasmic Reticulum Stress in MCF-7 Breast Cancer Cells.
Saleem MZ, Nisar MA, Alshwmi M, Din SRU, Gamallat Y, Khan M, Ma T
OncoTargets and therapy 2020;13:435-450
OncoTargets and therapy 2020;13:435-450
Inhibition of JNK-Mediated Autophagy Promotes Proscillaridin A- Induced Apoptosis via ROS Generation, Intracellular Ca+2 Oscillation and Inhibiting STAT3 Signaling in Breast Cancer Cells.
Saleem MZ, Alshwmi M, Zhang H, Din SRU, Nisar MA, Khan M, Alam S, Alam G, Jin L, Ma T
Frontiers in pharmacology 2020;11:01055
Frontiers in pharmacology 2020;11:01055
Shp2 activation in bone marrow microenvironment mediates the drug resistance of B-cell acute lymphoblastic leukemia through enhancing the role of VCAM-1/VLA-4.
Yu K, Yin Y, Ma D, Lu T, Wei D, Xiong J, Zhou Z, Zhang T, Zhang S, Fang Q, Wang J
International immunopharmacology 2020 Mar;80:106008
International immunopharmacology 2020 Mar;80:106008
miR-552 Regulates Liver Tumor-Initiating Cell Expansion and Sorafenib Resistance.
Han T, Zhang Y, Yang X, Han L, Li H, Chen T, Zheng Z
Molecular therapy. Nucleic acids 2020 Mar 6;19:1073-1085
Molecular therapy. Nucleic acids 2020 Mar 6;19:1073-1085
The role of tyrosine phosphatase Shp2 in spermatogonial differentiation and spermatocyte meiosis.
Li Y, Liu WS, Yi J, Kong SB, Ding JC, Zhao YN, Tian YP, Feng GS, Li CJ, Liu W, Wang HB, Lu ZX
Asian journal of andrology 2020 Jan-Feb;22(1):79-87
Asian journal of andrology 2020 Jan-Feb;22(1):79-87
Anticancer activity of dietary xanthone α-mangostin against hepatocellular carcinoma by inhibition of STAT3 signaling via stabilization of SHP1.
Zhang H, Tan YP, Zhao L, Wang L, Fu NJ, Zheng SP, Shen XF
Cell death & disease 2020 Jan 24;11(1):63
Cell death & disease 2020 Jan 24;11(1):63
Glycochenodeoxycholic acid induces stemness and chemoresistance via the STAT3 signaling pathway in hepatocellular carcinoma cells.
Shi C, Yang J, Hu L, Liao B, Qiao L, Shen W, Xie F, Zhu G
Aging 2020 Aug 3;12(15):15546-15555
Aging 2020 Aug 3;12(15):15546-15555
A thiazole-derived oridonin analogue exhibits antitumor activity by directly and allosterically inhibiting STAT3.
Shen X, Zhao L, Chen P, Gong Y, Liu D, Zhang X, Dai L, Sun Q, Lou J, Jin Z, Zhang B, Niu D, Chen C, Qi X, Jia D
The Journal of biological chemistry 2019 Nov 15;294(46):17471-17486
The Journal of biological chemistry 2019 Nov 15;294(46):17471-17486
Overexpression of miR-489 derails mammary hierarchy structure and inhibits HER2/neu-induced tumorigenesis.
Patel Y, Soni M, Awgulewitsch A, Kern MJ, Liu S, Shah N, Singh UP, Chen H
Oncogene 2019 Jan;38(3):445-453
Oncogene 2019 Jan;38(3):445-453
Proscillaridin A Promotes Oxidative Stress and ER Stress, Inhibits STAT3 Activation, and Induces Apoptosis in A549 Lung Adenocarcinoma Cells.
Maryam A, Mehmood T, Yan Q, Li Y, Khan M, Ma T
Oxidative medicine and cellular longevity 2018;2018:3853409
Oxidative medicine and cellular longevity 2018;2018:3853409
Proscillaridin A induces apoptosis, inhibits STAT3 activation and augments doxorubicin toxicity in prostate cancer cells.
He Y, Khan M, Yang J, Yao M, Yu S, Gao H
International journal of medical sciences 2018;15(8):832-839
International journal of medical sciences 2018;15(8):832-839
Chemical and genetic inhibition of STAT3 sensitizes hepatocellular carcinoma cells to sorafenib induced cell death.
Xie L, Zeng Y, Dai Z, He W, Ke H, Lin Q, Chen Y, Bu J, Lin D, Zheng M
International journal of biological sciences 2018;14(5):577-585
International journal of biological sciences 2018;14(5):577-585
The abnormal activation of D1R/Shp-2 complex involved in levodopa-induced dyskinesia in 6-hydroxydopamine-lesioned Parkinson's rats.
Wu N, Wan Y, Song L, Qi C, Liu Z, Gan J
Neuropsychiatric disease and treatment 2018;14:1779-1786
Neuropsychiatric disease and treatment 2018;14:1779-1786
Levodopa/Benserazide Loaded Microspheres Alleviate L-dopa Induced Dyskinesia through Preventing the Over-Expression of D1R/Shp-2/ERK1/2 Signaling Pathway in a Rat Model of Parkinson's Disease.
Wan Y, Wu N, Song L, Wang X, Liu Z, Yuan W, Gan J
Frontiers in aging neuroscience 2017;9:331
Frontiers in aging neuroscience 2017;9:331
Shp2 promotes liver cancer stem cell expansion by augmenting β-catenin signaling and predicts chemotherapeutic response of patients.
Xiang D, Cheng Z, Liu H, Wang X, Han T, Sun W, Li X, Yang W, Chen C, Xia M, Liu N, Yin S, Jin G, Lee T, Dong L, Hu H, Wang H, Ding J
Hepatology (Baltimore, Md.) 2017 May;65(5):1566-1580
Hepatology (Baltimore, Md.) 2017 May;65(5):1566-1580
Cryptotanshinone inhibits human glioma cell proliferation in vitro and in vivo through SHP-2-dependent inhibition of STAT3 activation.
Lu L, Zhang S, Li C, Zhou C, Li D, Liu P, Huang M, Shen X
Cell death & disease 2017 May 11;8(5):e2767
Cell death & disease 2017 May 11;8(5):e2767
Dietary quercetin potentiates the antiproliferative effect of interferon-α in hepatocellular carcinoma cells through activation of JAK/STAT pathway signaling by inhibition of SHP2 phosphatase.
Igbe I, Shen XF, Jiao W, Qiang Z, Deng T, Li S, Liu WL, Liu HW, Zhang GL, Wang F
Oncotarget 2017 Dec 26;8(69):113734-113748
Oncotarget 2017 Dec 26;8(69):113734-113748
Expression and clinical significance of tyrosine phosphatase SHP2 in thyroid carcinoma.
Hu ZQ, Ma R, Zhang CM, Li J, Li L, Hu ZT, Gao QI, Li WM
Oncology letters 2015 Sep;10(3):1507-1512
Oncology letters 2015 Sep;10(3):1507-1512
Chemical composition of total flavonoids from Polygonum amplexicaule and their pro-apoptotic effect on hepatocellular carcinoma cells: Potential roles of suppressing STAT3 signaling.
Xiang M, Su H, Hong Z, Yang T, Shu G
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2015 Jun;80:62-71
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2015 Jun;80:62-71
Systemic lupus erythematosus immune complexes increase the expression of SLAM family members CD319 (CRACC) and CD229 (LY-9) on plasmacytoid dendritic cells and CD319 on CD56(dim) NK cells.
Hagberg N, Theorell J, Schlums H, Eloranta ML, Bryceson YT, Rönnblom L
Journal of immunology (Baltimore, Md. : 1950) 2013 Sep 15;191(6):2989-98
Journal of immunology (Baltimore, Md. : 1950) 2013 Sep 15;191(6):2989-98
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 20145-1-AP(PTPN11 antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using PTPN11 antibody 20145-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human breast cancer using 20145-1-AP(PTPN11 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human breast cancer using 20145-1-AP(PTPN11 antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue
