EB05114

antibody from Everest Biotech

Targeting: PTPN11 BPTP3, NS1, PTP2C, SH-PTP2, SHP-2, SHP2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

EB05114

Provider

Everest Biotech

Proper citation

Everest Biotech Cat#EB05114, RRID:AB_2175075

Product name

Goat anti-SHP2 / PTPN11 Antibody

Antibody type

Polyclonal

Description

Goat anti-SHP2 / PTPN11 Antibody

Reactivity

Human

Host

Goat

Antigen sequence

C-YENVGLMQQQKSFR


Epitope

C Terminus

Isotype

IgG

Vial size

100µg specific antibody in 200µl

Concentration

0.5 mg/ml

Storage

Aliquot and store at -20°C. Minimize freezing and thawing.

Western blot

Supportive validation

Submitted by

Everest Biotech (provider)

Main image

Experimental details

EB05114 staining (2µg/ml) of human muscle lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Supportive validation

Submitted by

Everest Biotech (provider)

Main image

Experimental details

HEK293 overexpressing PTPN11 (RC220029) and probed with EB05114 (mock transfection in first lane), tested by Origene.

Immunohistochemistry

Supportive validation

Submitted by

Everest Biotech (provider)

Main image

Experimental details

EB05114 (3.8µg/ml) staining of paraffin embedded Human Cerebellum Steamed antigen retrieval with citrate buffer pH 6, AP-staining.