M00150-2

antibody from Boster Biological Technology

Targeting: PTPN11 BPTP3, NS1, PTP2C, SH-PTP2, SHP-2, SHP2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

M00150-2

Provider

Boster Biological Technology

Product name

Anti-SHP2/PTPN11 Antibody Picoband™ (monoclonal, 2E6)

Antibody type

Monoclonal

Description

Mouse IgG monoclonal antibody for SHP2/PTPN11 detection. Tested with WB, IHC-P, ICC/IF, FCM in Human;Mouse;Rat.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

2.00E+06

Vial size

100μg/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of SHP2/PTPN11 using anti-SHP2/PTPN11 antibody (M00150-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human CCRF-CEM whole cell lysates. Lane 4: human K562 whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human CACO-2 whole cell lysates, Lane 7: human Hela whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: rat C6 whole cell lysates, Lane 10: mouse brain tissue lysates, Lane 11: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SHP2/PTPN11 antigen affinity purified monoclonal antibody (Catalog # M00150-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SHP2/PTPN11 at approximately 70KD. The expected band size for SHP2/PTPN11 is at 70KD.

Additional image