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Learn20145-1-AP
antibody from Invitrogen Antibodies
Targeting: PTPN11
BPTP3, NS1, PTP2C, SH-PTP2, SHP-2, SHP2
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [3]
- Immunohistochemistry [7]
- Other assay [1]
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- Product number
- 20145-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SHP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MTSRRWFHP NITGVEAENL LLTRGVDGSF LARPSKSNPG DFTLSVRRNG AVTHIKIQNT GDYYDLYGGE KFATLAELVQ YYMEHHGQLK EKNGDVIELK YPLNCADPTS ERWFHGHLSG KEAEKLLTEK GKHGSFLVRE SQSHPGDFVL SVRTGDDKGE SNDGKSKVTH VMIRCQELKY DVGGGERFDS LTDLVEHYKK NPMVETLGTV LQLKQPLNTT RINAAEIESR VRELSKLAET TDKVKQGFWE EFETLQQQEC KLLYSRKEGQ RQENKNKNRY KNILPFDHTR VVLHDGDPNE PVSDYINANI IMPEFETKCN NSKPKKSYIA TQGCLQNTVN DFWRMVFQEN SRVIVMTT (1-357 aa encoded by BC008692)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.23 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 20145-1-AP (SHP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20145-1-AP (SHP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- U-937 cells were subjected to SDS PAGE followed by western blot with 20145-1-AP (SHP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A431 cells were subjected to SDS PAGE followed by western blot with 20145-1-AP (SHP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using PTPN11 antibody 20145-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using PTPN11 antibody 20145-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using PTPN11 antibody 20145-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 20145-1-AP (SHP2 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 20145-1-AP (SHP2 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer using 20145-1-AP (SHP2 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer using 20145-1-AP (SHP2 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 20145-1-AP ( SHP2 antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 20145-1-AP ( SHP2 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using (Product # 20145-1-AP) (SHP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-SHP2 (IP:20145-1-AP, 4ug; Detection:20145-1-AP 1:1000) with HEK-293 cells lysate 2800ug.
