Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-806 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-806, RRID:AB_2284763
- Product name
- Goat Polyclonal SHP-2/PTPN11 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references The SH2 tyrosine phosphatase shp2 is required for mammalian limb development.
Saxton TM, Ciruna BG, Holmyard D, Kulkarni S, Harpal K, Rossant J, Pawson T
Nature genetics 2000 Apr;24(4):420-3
Nature genetics 2000 Apr;24(4):420-3
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SHP-2/PTPN11 Antibody [NB100-806] - Human muscle lysate (RIPA buffer, 35 ug total protein). Primary antibody at 2 ug/ml, 1 hr incubation. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SHP-2/PTPN11 Antibody [NB100-806] - HEK293 overexpressing PTPN11 and probed with (mock transfection in first lane).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SHP-2/PTPN11 Antibody [NB100-806] - Human Muscle lysate (RIPA buffer, 35 ug total protein per lane). Antibody at 2 ug/mL. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SHP-2/PTPN11 Antibody [NB100-806] - (3.8ug/ml) staining of paraffin embedded Human Cerebellum Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: SHP-2/PTPN11 Antibody [NB100-806] - Analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
