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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [1]
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- Product number
- AF3515 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Nogo-A aa 566-748 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Nogo-A in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant rat Nogo-A (aa 543-725) and recombinant human Nogo-B is observed..
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q9NQC3- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Histamine Receptor 3 negatively regulates oligodendrocyte differentiation and remyelination.
Protein microarray analysis identifies cyclic nucleotide phosphodiesterase as an interactor of Nogo-A.
Chen Y, Zhen W, Guo T, Zhao Y, Liu A, Rubio JP, Krull D, Richardson JC, Lu H, Wang R
PloS one 2017;12(12):e0189380
PloS one 2017;12(12):e0189380
Protein microarray analysis identifies cyclic nucleotide phosphodiesterase as an interactor of Nogo-A.
Sumiyoshi K, Obayashi S, Tabunoki H, Arima K, Satoh J
Neuropathology : official journal of the Japanese Society of Neuropathology 2010 Feb 1;30(1):7-14
Neuropathology : official journal of the Japanese Society of Neuropathology 2010 Feb 1;30(1):7-14
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Nogo-A in Rat Cortical Stem Cells. Nogo-A was detected in immersion fixed 7 day differentiated rat cortical stem cells using 10 µg/mL Sheep Anti-Human Nogo-A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3515) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
