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- References [13]
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- Immunohistochemistry [1]
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- Product number
- NB100-56681 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56681, RRID:AB_838641
- Product name
- Rabbit Polyclonal Nogo Antibody
- Antibody type
- Polyclonal
- Description
- Protein G purified. This recognizes Nogo-A (approx. 220 kD)and Nogo-B2 and B1 (approx. 50 kD and 48 kD in human and 43 kD and 41 kD in mouse and rat, respectively).
- Reactivity
- Human, Mouse, Rat, Rabbit, Sheep
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.2 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Rosiglitazone alleviates intrahepatic cholestasis induced by α-naphthylisothiocyanate in mice: The role of circulating 15-deoxy-Δ(12,14) -PGJ(2) and Nogo.
Intrinsically Disordered Protein TEX264 Mediates ER-phagy.
Feedback-Driven Mechanisms between Microtubules and the Endoplasmic Reticulum Instruct Neuronal Polarity.
Nogo-B receptor increases the resistance to tamoxifen in estrogen receptor-positive breast cancer cells.
Nogo-B (Reticulon-4B) functions as a negative regulator of the apoptotic pathway through the interaction with c-FLIP in colorectal cancer cells.
Nogo-B receptor increases the resistance of estrogen receptor positive breast cancer to paclitaxel.
Reticulon-4B/Nogo-B acts as a molecular linker between microtubules and actin cytoskeleton in vascular smooth muscle cells.
Reticulon 4 is necessary for endoplasmic reticulum tubulation, STIM1-Orai1 coupling, and store-operated calcium entry.
BioPEGylation of polyhydroxybutyrate promotes nerve cell health and migration.
Nogo-B receptor modulates angiogenesis response of pulmonary artery endothelial cells through eNOS coupling.
Expression of NgBR is highly associated with estrogen receptor alpha and survivin in breast cancer.
Comparative proteomics profiling of a phospholamban mutant mouse model of dilated cardiomyopathy reveals progressive intracellular stress responses.
Low levels of Nogo-B in human carotid atherosclerotic plaques are associated with an atheromatous phenotype, restenosis, and stenosis severity.
Zhang S, Yu M, Guo F, Yang X, Chen Y, Ma C, Li Q, Wei Z, Li X, Wang H, Hu H, Zhang Y, Kong D, Miao QR, Hu W, Hajjar DP, Zhu Y, Han J, Duan Y
British journal of pharmacology 2020 Mar;177(5):1041-1060
British journal of pharmacology 2020 Mar;177(5):1041-1060
Intrinsically Disordered Protein TEX264 Mediates ER-phagy.
Chino H, Hatta T, Natsume T, Mizushima N
Molecular cell 2019 Jun 6;74(5):909-921.e6
Molecular cell 2019 Jun 6;74(5):909-921.e6
Feedback-Driven Mechanisms between Microtubules and the Endoplasmic Reticulum Instruct Neuronal Polarity.
Farías GG, Fréal A, Tortosa E, Stucchi R, Pan X, Portegies S, Will L, Altelaar M, Hoogenraad CC
Neuron 2019 Apr 3;102(1):184-201.e8
Neuron 2019 Apr 3;102(1):184-201.e8
Nogo-B receptor increases the resistance to tamoxifen in estrogen receptor-positive breast cancer cells.
Gao P, Wang X, Jin Y, Hu W, Duan Y, Shi A, Du Y, Song D, Yang M, Li S, Han B, Zhao G, Zhang H, Fan Z, Miao QR
Breast cancer research : BCR 2018 Sep 12;20(1):112
Breast cancer research : BCR 2018 Sep 12;20(1):112
Nogo-B (Reticulon-4B) functions as a negative regulator of the apoptotic pathway through the interaction with c-FLIP in colorectal cancer cells.
Kawaguchi N, Tashiro K, Taniguchi K, Kawai M, Tanaka K, Okuda J, Hayashi M, Uchiyama K
Biochimica et biophysica acta. Molecular basis of disease 2018 Aug;1864(8):2600-2609
Biochimica et biophysica acta. Molecular basis of disease 2018 Aug;1864(8):2600-2609
Nogo-B receptor increases the resistance of estrogen receptor positive breast cancer to paclitaxel.
Jin Y, Hu W, Liu T, Rana U, Aguilera-Barrantes I, Kong A, Kumar SN, Wang B, Gao P, Wang X, Duan Y, Shi A, Song D, Yang M, Li S, Han B, Zhao G, Fan Z, Miao QR
Cancer letters 2018 Apr 10;419:233-244
Cancer letters 2018 Apr 10;419:233-244
Reticulon-4B/Nogo-B acts as a molecular linker between microtubules and actin cytoskeleton in vascular smooth muscle cells.
Rodríguez-Feo JA, Gallego-Delgado J, Puerto M, Wandosell F, Osende J
Biochimica et biophysica acta 2016 Aug;1863(8):1985-95
Biochimica et biophysica acta 2016 Aug;1863(8):1985-95
Reticulon 4 is necessary for endoplasmic reticulum tubulation, STIM1-Orai1 coupling, and store-operated calcium entry.
Jozsef L, Tashiro K, Kuo A, Park EJ, Skoura A, Albinsson S, Rivera-Molina F, Harrison KD, Iwakiri Y, Toomre D, Sessa WC
The Journal of biological chemistry 2014 Mar 28;289(13):9380-95
The Journal of biological chemistry 2014 Mar 28;289(13):9380-95
BioPEGylation of polyhydroxybutyrate promotes nerve cell health and migration.
Chan RT, Russell RA, Marçal H, Lee TH, Holden PJ, Foster LJ
Biomacromolecules 2014 Jan 13;15(1):339-49
Biomacromolecules 2014 Jan 13;15(1):339-49
Nogo-B receptor modulates angiogenesis response of pulmonary artery endothelial cells through eNOS coupling.
Teng RJ, Rana U, Afolayan AJ, Zhao B, Miao QR, Konduri GG
American journal of respiratory cell and molecular biology 2014 Aug;51(2):169-77
American journal of respiratory cell and molecular biology 2014 Aug;51(2):169-77
Expression of NgBR is highly associated with estrogen receptor alpha and survivin in breast cancer.
Wang B, Zhao B, North P, Kong A, Huang J, Miao QR
PloS one 2013;8(11):e78083
PloS one 2013;8(11):e78083
Comparative proteomics profiling of a phospholamban mutant mouse model of dilated cardiomyopathy reveals progressive intracellular stress responses.
Gramolini AO, Kislinger T, Alikhani-Koopaei R, Fong V, Thompson NJ, Isserlin R, Sharma P, Oudit GY, Trivieri MG, Fagan A, Kannan A, Higgins DG, Huedig H, Hess G, Arab S, Seidman JG, Seidman CE, Frey B, Perry M, Backx PH, Liu PP, MacLennan DH, Emili A
Molecular & cellular proteomics : MCP 2008 Mar;7(3):519-33
Molecular & cellular proteomics : MCP 2008 Mar;7(3):519-33
Low levels of Nogo-B in human carotid atherosclerotic plaques are associated with an atheromatous phenotype, restenosis, and stenosis severity.
Rodriguez-Feo JA, Hellings WE, Verhoeven BA, Moll FL, de Kleijn DP, Prendergast J, Gao Y, van der Graaf Y, Tellides G, Sessa WC, Pasterkamp G
Arteriosclerosis, thrombosis, and vascular biology 2007 Jun;27(6):1354-60
Arteriosclerosis, thrombosis, and vascular biology 2007 Jun;27(6):1354-60
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Nogo Antibody [NB100-56681] - Lane view shows a specific band for Nogo in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nogo Antibody [NB100-56681] - Analysis of Nogo A and B in A) human, B) mouse, and C) rat brain tissue lysate using Nogo antibody at 1:2000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nogo Antibody [NB100-56681] - Analysis of human testis using Nogo antibody at 1:100.
