Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-36771 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-SMAD1 (Ser465) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 60 kDa. Purity is >95% by SDS-PAGE.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Elevated IgE promotes cardiac fibrosis by suppressing miR-486a-5p.
Zhao H, Yang H, Geng C, Chen Y, Tang Y, Li Z, Pang J, Shu T, Nie Y, Liu Y, Jia K, Wang J
Theranostics 2021;11(15):7600-7615
Theranostics 2021;11(15):7600-7615
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-SMAD1 pSer465 using Phospho-SMAD1 pSer465 polyclonal antibody (Product # PA5-36771) at a dilution of 1:500. Lane 1: THP-1 cell lysate treated with serum (10%, 1h), Lane 2: sp2/0 cell lysate treated with serum (10%, 1h).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-SMAD1 (Ser465) in Lane 1: A549 whole cell lysate (40 µg), Lane 2: A549 treated with UV for 5 minutes then repair for 1 hour whole cell lysate (40 µg), Lane 3: A549 treated with UV for 5 minutes then repair for 3 hours whole cell lysate (40 µg), Lane 4: A549 treated with UV for 5 minutes then repair for 6 hours whole cell lysate (40 µg), Lane 5: A549 treated with UV for 5 minutes then repair for 24 hours whole cell lysate (40 µg), Lane 6: the Uterus tissue lysate of mouse (40 µg), Lane 7: the Uterus tissue lysate of rat (40 µg). Samples were incubated with Phospho-SMAD1 (Ser465) polyclonal antibody (Product # PA5-36771) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-SMAD1 (Ser465) in paraffin-embedded human colorectal carcinoma tissue. Samples were incubated with Phospho-SMAD1 (Ser465) polyclonal antibody (Product # PA5-36771) at a dilution of 1:50.