Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-36876 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-SMAD1 (Ser187) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 60 kDa. Purity is >95% by SDS-PAGE.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-SMAD1 pSer187 using Phospho-SMAD1 pSer187 polyclonal antibody (Product # PA5-36876) at a dilution of 1:500. Lane 1: Hela cell lysate treated with UV (4h), Lane 2: Raw264.7 cell lysate treated with UV (4h), Lane 3: H9C2 cell lysate treated with UV (4h).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-SMAD1 pSer187 using Phospho-SMAD1 pSer187 polyclonal antibody (Product # PA5-36876) at a dilution of 1:500. Lane 1: Hela cell lysate treated with UV (4h), Lane 2: Raw264.7 cell lysate treated with UV (4h), Lane 3: H9C2 cell lysate treated with UV (4h).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-SMAD1 (Ser187) in Lane 1: HeLa cell lysate treated with UV (4h), Lane 2: Raw264.7 cell lysate treated with UV (4h), Lane 3: H9C2 cell lysate treated with UV (4h). Samples were incubated with Phospho-SMAD1 (Ser187) polyclonal antibody (Product # PA5-36876) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-SMAD1 (Ser187) in paraffin-embedded human breast carcinoma tissue (cytoplasmic and nucleus staining) and negative control (right, with PBS only). Samples were incubated with Phospho-SMAD1 (Ser187) polyclonal antibody (Product # PA5-36876) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.