Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00004086-M03 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00004086-M03, RRID:AB_715084
- Product name
- Mouse Monoclonal Smad1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. SMAD1 (2E9)
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Temporal and regional patterns of Smad activation in the rat hippocampus following global ischemia.
Nakajima T, Yanagihara M, Nishii H
Journal of the neurological sciences 2014 Feb 15;337(1-2):25-37
Journal of the neurological sciences 2014 Feb 15;337(1-2):25-37
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Smad1 Antibody (2E9) [H00004086-M03] - Analysis of SMAD1 expression in IMR-32 (Cat # L008V1).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Smad1 Antibody (2E9) [H00004086-M03] - Analysis of SMAD1 expression in HeLa (Cat # L013V1).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Smad1 Antibody (2E9) [H00004086-M03] - Detection limit for recombinant GST tagged SMAD1 is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Smad1 Antibody (2E9) [H00004086-M03] - Analysis of monoclonal antibody to SMAD1 on HeLa cell. Antibody concentration 10 ug/ml
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Smad1 Antibody (2E9) [H00004086-M03] - Analysis of monoclonal antibody to SMAD1 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml