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- Antibody Data
- Antigen structure
- References [28]
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- Validations
- Immunocytochemistry [1]
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- Product number
- BML-PW8810-0100 - Provider product page
- Provider
- Enzo Life Sciences
- Product name
- Mono- and polyubiquitinylated conjugates monoclonal antibody (FK2)
- Antibody type
- Monoclonal
- Antigen
- Purified from natural sources
- Description
- Purified.
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- FK2
- Vial size
- 100 μg
- Storage
- -20°C
- Handling
- Store unopened vial at -20¡C until required for use. Store diluted antibody at 2-4¡C and use within 1 month. Avoid freeze/thaw cycles.
Submitted references Npas4 Transcription Factor Expression Is Regulated by Calcium Signaling Pathways and Prevents Tacrolimus-induced Cytotoxicity in Pancreatic Beta Cells.
USP7 is essential for maintaining Rad18 stability and DNA damage tolerance.
Mitotic phosphorylation of Bloom helicase at Thr182 is required for its proteasomal degradation and maintenance of chromosomal stability.
Histone H1 couples initiation and amplification of ubiquitin signalling after DNA damage.
Aneuploidy causes premature differentiation of neural and intestinal stem cells.
Effects of resveratrol on growth and skeletal muscle physiology of juvenile southern flounder.
A Point Mutation in the Ubiquitin Ligase RNF170 That Causes Autosomal Dominant Sensory Ataxia Destabilizes the Protein and Impairs Inositol 1,4,5-Trisphosphate Receptor-mediated Ca2+ Signaling.
BAF is a cytosolic DNA sensor that leads to exogenous DNA avoiding autophagy.
Bortezomib Amplifies Effect on Intracellular Proteasomes by Changing Proteasome Structure.
Pompe disease results in a Golgi-based glycosylation deficit in human induced pluripotent stem cell-derived cardiomyocytes.
Keap1 regulates inflammatory signaling in Mycobacterium avium-infected human macrophages.
p16 Protein and gigaxonin are associated with the ubiquitination of NFκB in cisplatin-induced senescence of cancer cells.
Activation of the ubiquitin-proteasome system against arsenic trioxide cardiotoxicity involves ubiquitin ligase Parkin for mitochondrial homeostasis.
Distinct effects of methamphetamine on autophagy-lysosome and ubiquitin-proteasome systems in HL-1 cultured mouse atrial cardiomyocytes.
Heterologous SUMO-2/3-ubiquitin chains optimize IκBα degradation and NF-κB activity.
The proteasomal de-ubiquitinating enzyme POH1 promotes the double-strand DNA break response.
Autophagy inhibition promotes defective neosynthesized proteins storage in ALIS, and induces redirection toward proteasome processing and MHCI-restricted presentation.
Hectd1 regulates intracellular localization and secretion of Hsp90 to control cellular behavior of the cranial mesenchyme.
Dynamics of the degradation of ubiquitinated proteins by proteasomes and autophagy: association with sequestosome 1/p62.
Immunoreactivity to Lys63-linked polyubiquitin is a feature of neurodegeneration.
Loss of hrs in the central nervous system causes accumulation of ubiquitinated proteins and neurodegeneration.
Large-scale analysis of the human ubiquitin-related proteome.
MHC class I ubiquitination by a viral PHD/LAP finger protein.
Isolation of ubiquitin-E2 (ubiquitin-conjugating enzyme) complexes from erythroleukaemia cells using immunoaffinity techniques.
Chemotropic responses of retinal growth cones mediated by rapid local protein synthesis and degradation.
Dynamics of ubiquitin conjugation during heat-shock response revealed by using a monoclonal antibody specific to multi-ubiquitin chains.
Immunoassay for the quantification of intracellular multi-ubiquitin chains.
Production and characterization of monoclonal antibodies specific to multi-ubiquitin chains of polyubiquitinated proteins.
Speckmann T, Sabatini PV, Nian C, Smith RG, Lynn FC
The Journal of biological chemistry 2016 Feb 5;291(6):2682-95
The Journal of biological chemistry 2016 Feb 5;291(6):2682-95
USP7 is essential for maintaining Rad18 stability and DNA damage tolerance.
Zlatanou A, Sabbioneda S, Miller ES, Greenwalt A, Aggathanggelou A, Maurice MM, Lehmann AR, Stankovic T, Reverdy C, Colland F, Vaziri C, Stewart GS
Oncogene 2016 Feb 25;35(8):965-76
Oncogene 2016 Feb 25;35(8):965-76
Mitotic phosphorylation of Bloom helicase at Thr182 is required for its proteasomal degradation and maintenance of chromosomal stability.
Kharat SS, Tripathi V, Damodaran AP, Priyadarshini R, Chandra S, Tikoo S, Nandhakumar R, Srivastava V, Priya S, Hussain M, Kaur S, Fishman JB, Sengupta S
Oncogene 2016 Feb 25;35(8):1025-38
Oncogene 2016 Feb 25;35(8):1025-38
Histone H1 couples initiation and amplification of ubiquitin signalling after DNA damage.
Thorslund T, Ripplinger A, Hoffmann S, Wild T, Uckelmann M, Villumsen B, Narita T, Sixma TK, Choudhary C, Bekker-Jensen S, Mailand N
Nature 2015 Nov 19;527(7578):389-93
Nature 2015 Nov 19;527(7578):389-93
Aneuploidy causes premature differentiation of neural and intestinal stem cells.
Gogendeau D, Siudeja K, Gambarotto D, Pennetier C, Bardin AJ, Basto R
Nature communications 2015 Nov 17;6:8894
Nature communications 2015 Nov 17;6:8894
Effects of resveratrol on growth and skeletal muscle physiology of juvenile southern flounder.
Wilson WN, Baumgarner BL, Watanabe WO, Alam MS, Kinsey ST
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 2015 May;183:27-35
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 2015 May;183:27-35
A Point Mutation in the Ubiquitin Ligase RNF170 That Causes Autosomal Dominant Sensory Ataxia Destabilizes the Protein and Impairs Inositol 1,4,5-Trisphosphate Receptor-mediated Ca2+ Signaling.
Wright FA, Lu JP, Sliter DA, Dupré N, Rouleau GA, Wojcikiewicz RJ
The Journal of biological chemistry 2015 May 29;290(22):13948-57
The Journal of biological chemistry 2015 May 29;290(22):13948-57
BAF is a cytosolic DNA sensor that leads to exogenous DNA avoiding autophagy.
Kobayashi S, Koujin T, Kojidani T, Osakada H, Mori C, Hiraoka Y, Haraguchi T
Proceedings of the National Academy of Sciences of the United States of America 2015 Jun 2;112(22):7027-32
Proceedings of the National Academy of Sciences of the United States of America 2015 Jun 2;112(22):7027-32
Bortezomib Amplifies Effect on Intracellular Proteasomes by Changing Proteasome Structure.
Pitcher DS, de Mattos-Shipley K, Tzortzis K, Auner HW, Karadimitris A, Kleijnen MF
EBioMedicine 2015 Jul;2(7):642-8
EBioMedicine 2015 Jul;2(7):642-8
Pompe disease results in a Golgi-based glycosylation deficit in human induced pluripotent stem cell-derived cardiomyocytes.
Raval KK, Tao R, White BE, De Lange WJ, Koonce CH, Yu J, Kishnani PS, Thomson JA, Mosher DF, Ralphe JC, Kamp TJ
The Journal of biological chemistry 2015 Jan 30;290(5):3121-36
The Journal of biological chemistry 2015 Jan 30;290(5):3121-36
Keap1 regulates inflammatory signaling in Mycobacterium avium-infected human macrophages.
Awuh JA, Haug M, Mildenberger J, Marstad A, Do CP, Louet C, Stenvik J, Steigedal M, Damås JK, Halaas Ø, Flo TH
Proceedings of the National Academy of Sciences of the United States of America 2015 Aug 4;112(31):E4272-80
Proceedings of the National Academy of Sciences of the United States of America 2015 Aug 4;112(31):E4272-80
p16 Protein and gigaxonin are associated with the ubiquitination of NFκB in cisplatin-induced senescence of cancer cells.
Veena MS, Wilken R, Zheng JY, Gholkar A, Venkatesan N, Vira D, Ahmed S, Basak SK, Dalgard CL, Ravichandran S, Batra RK, Kasahara N, Elashoff D, Fishbein MC, Whitelegge JP, Torres JZ, Wang MB, Srivatsan ES
The Journal of biological chemistry 2014 Dec 12;289(50):34921-37
The Journal of biological chemistry 2014 Dec 12;289(50):34921-37
Activation of the ubiquitin-proteasome system against arsenic trioxide cardiotoxicity involves ubiquitin ligase Parkin for mitochondrial homeostasis.
Watanabe M, Funakoshi T, Unuma K, Aki T, Uemura K
Toxicology 2014 Aug 1;322:43-50
Toxicology 2014 Aug 1;322:43-50
Distinct effects of methamphetamine on autophagy-lysosome and ubiquitin-proteasome systems in HL-1 cultured mouse atrial cardiomyocytes.
Funakoshi-Hirose I, Aki T, Unuma K, Funakoshi T, Noritake K, Uemura K
Toxicology 2013 Oct 4;312:74-82
Toxicology 2013 Oct 4;312:74-82
Heterologous SUMO-2/3-ubiquitin chains optimize IκBα degradation and NF-κB activity.
Aillet F, Lopitz-Otsoa F, Egaña I, Hjerpe R, Fraser P, Hay RT, Rodriguez MS, Lang V
PloS one 2012;7(12):e51672
PloS one 2012;7(12):e51672
The proteasomal de-ubiquitinating enzyme POH1 promotes the double-strand DNA break response.
Butler LR, Densham RM, Jia J, Garvin AJ, Stone HR, Shah V, Weekes D, Festy F, Beesley J, Morris JR
The EMBO journal 2012 Oct 3;31(19):3918-34
The EMBO journal 2012 Oct 3;31(19):3918-34
Autophagy inhibition promotes defective neosynthesized proteins storage in ALIS, and induces redirection toward proteasome processing and MHCI-restricted presentation.
Wenger T, Terawaki S, Camosseto V, Abdelrassoul R, Mies A, Catalan N, Claudio N, Clavarino G, de Gassart A, Rigotti Fde A, Gatti E, Pierre P
Autophagy 2012 Mar;8(3):350-63
Autophagy 2012 Mar;8(3):350-63
Hectd1 regulates intracellular localization and secretion of Hsp90 to control cellular behavior of the cranial mesenchyme.
Sarkar AA, Zohn IE
The Journal of cell biology 2012 Mar 19;196(6):789-800
The Journal of cell biology 2012 Mar 19;196(6):789-800
Dynamics of the degradation of ubiquitinated proteins by proteasomes and autophagy: association with sequestosome 1/p62.
Myeku N, Figueiredo-Pereira ME
The Journal of biological chemistry 2011 Jun 24;286(25):22426-40
The Journal of biological chemistry 2011 Jun 24;286(25):22426-40
Immunoreactivity to Lys63-linked polyubiquitin is a feature of neurodegeneration.
Paine S, Bedford L, Thorpe JR, Mayer RJ, Cavey JR, Bajaj N, Sheppard PW, Lowe J, Layfield R
Neuroscience letters 2009 Sep 4;460(3):205-8
Neuroscience letters 2009 Sep 4;460(3):205-8
Loss of hrs in the central nervous system causes accumulation of ubiquitinated proteins and neurodegeneration.
Tamai K, Toyoshima M, Tanaka N, Yamamoto N, Owada Y, Kiyonari H, Murata K, Ueno Y, Ono M, Shimosegawa T, Yaegashi N, Watanabe M, Sugamura K
The American journal of pathology 2008 Dec;173(6):1806-17
The American journal of pathology 2008 Dec;173(6):1806-17
Large-scale analysis of the human ubiquitin-related proteome.
Matsumoto M, Hatakeyama S, Oyamada K, Oda Y, Nishimura T, Nakayama KI
Proteomics 2005 Nov;5(16):4145-51
Proteomics 2005 Nov;5(16):4145-51
MHC class I ubiquitination by a viral PHD/LAP finger protein.
Boname JM, Stevenson PG
Immunity 2001 Oct;15(4):627-36
Immunity 2001 Oct;15(4):627-36
Isolation of ubiquitin-E2 (ubiquitin-conjugating enzyme) complexes from erythroleukaemia cells using immunoaffinity techniques.
Takada K, Hirakawa T, Yokosawa H, Okawa Y, Taguchi H, Ohkawa K
The Biochemical journal 2001 May 15;356(Pt 1):199-206
The Biochemical journal 2001 May 15;356(Pt 1):199-206
Chemotropic responses of retinal growth cones mediated by rapid local protein synthesis and degradation.
Campbell DS, Holt CE
Neuron 2001 Dec 20;32(6):1013-26
Neuron 2001 Dec 20;32(6):1013-26
Dynamics of ubiquitin conjugation during heat-shock response revealed by using a monoclonal antibody specific to multi-ubiquitin chains.
Fujimuro M, Sawada H, Yokosawa H
European journal of biochemistry / FEBS 1997 Oct 15;249(2):427-33
European journal of biochemistry / FEBS 1997 Oct 15;249(2):427-33
Immunoassay for the quantification of intracellular multi-ubiquitin chains.
Takada K, Nasu H, Hibi N, Tsukada Y, Ohkawa K, Fujimuro M, Sawada H, Yokosawa H
European journal of biochemistry / FEBS 1995 Oct 1;233(1):42-7
European journal of biochemistry / FEBS 1995 Oct 1;233(1):42-7
Production and characterization of monoclonal antibodies specific to multi-ubiquitin chains of polyubiquitinated proteins.
Fujimuro M, Sawada H, Yokosawa H
FEBS letters 1994 Aug 1;349(2):173-80
FEBS letters 1994 Aug 1;349(2):173-80
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot of multi-ubiquitin chains using MAb to Polyubiquitinylated Conjugates (FK1) (Prod. No. BML-PW8805) (lanes A-C) and MAb to Mono- and Polyubiquitinylated Conjugates (FK2) (Prod. No. BML-PW8810) (lanes D-F).Lanes A & D: K48-linked chains.Lanes B & E: K29-linked chains. Lanes C & F: K63-linked chains.
