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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-8304 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-8304, RRID:AB_2214058
- Product name
- Anti-VCAM1
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
Submitted references Neutrophil adhesion on endothelial cells in a novel asymmetric stenosis model: effect of wall shear stress gradients.
Oxidized hemoglobin is an endogenous proinflammatory agonist that targets vascular endothelial cells.
Nuclear factor-kappaB enhances ErbB2-induced mammary tumorigenesis and neoangiogenesis in vivo.
Differentiated murine airway epithelial cells synthesize a leukocyte-adhesive hyaluronan matrix in response to endoplasmic reticulum stress.
Human mesenchymal stem cells at the single-cell level: simultaneous seven-colour immunofluorescence.
Pulmonary CXCR2 regulates VCAM-1 and antigen-induced recruitment of mast cell progenitors.
A homing mechanism for bone marrow-derived progenitor cell recruitment to the neovasculature.
Ets-1 is a critical regulator of Ang II-mediated vascular inflammation and remodeling.
Integrin alpha4beta1-VCAM-1-mediated adhesion between endothelial and mural cells is required for blood vessel maturation.
Rouleau L, Copland IB, Tardif JC, Mongrain R, Leask RL
Annals of biomedical engineering 2010 Sep;38(9):2791-804
Annals of biomedical engineering 2010 Sep;38(9):2791-804
Oxidized hemoglobin is an endogenous proinflammatory agonist that targets vascular endothelial cells.
Silva G, Jeney V, Chora A, Larsen R, Balla J, Soares MP
The Journal of biological chemistry 2009 Oct 23;284(43):29582-95
The Journal of biological chemistry 2009 Oct 23;284(43):29582-95
Nuclear factor-kappaB enhances ErbB2-induced mammary tumorigenesis and neoangiogenesis in vivo.
Liu M, Ju X, Willmarth NE, Casimiro MC, Ojeifo J, Sakamaki T, Katiyar S, Jiao X, Popov VM, Yu Z, Wu K, Joyce D, Wang C, Pestell RG
The American journal of pathology 2009 May;174(5):1910-20
The American journal of pathology 2009 May;174(5):1910-20
Differentiated murine airway epithelial cells synthesize a leukocyte-adhesive hyaluronan matrix in response to endoplasmic reticulum stress.
Lauer ME, Erzurum SC, Mukhopadhyay D, Vasanji A, Drazba J, Wang A, Fulop C, Hascall VC
The Journal of biological chemistry 2008 Sep 19;283(38):26283-96
The Journal of biological chemistry 2008 Sep 19;283(38):26283-96
Human mesenchymal stem cells at the single-cell level: simultaneous seven-colour immunofluorescence.
Schieker M, Pautke C, Haasters F, Schieker J, Docheva D, Böcker W, Guelkan H, Neth P, Jochum M, Mutschler W
Journal of anatomy 2007 May;210(5):592-9
Journal of anatomy 2007 May;210(5):592-9
Pulmonary CXCR2 regulates VCAM-1 and antigen-induced recruitment of mast cell progenitors.
Hallgren J, Jones TG, Abonia JP, Xing W, Humbles A, Austen KF, Gurish MF
Proceedings of the National Academy of Sciences of the United States of America 2007 Dec 18;104(51):20478-83
Proceedings of the National Academy of Sciences of the United States of America 2007 Dec 18;104(51):20478-83
A homing mechanism for bone marrow-derived progenitor cell recruitment to the neovasculature.
Jin H, Aiyer A, Su J, Borgstrom P, Stupack D, Friedlander M, Varner J
The Journal of clinical investigation 2006 Mar;116(3):652-62
The Journal of clinical investigation 2006 Mar;116(3):652-62
Ets-1 is a critical regulator of Ang II-mediated vascular inflammation and remodeling.
Zhan Y, Brown C, Maynard E, Anshelevich A, Ni W, Ho IC, Oettgen P
The Journal of clinical investigation 2005 Sep;115(9):2508-16
The Journal of clinical investigation 2005 Sep;115(9):2508-16
Integrin alpha4beta1-VCAM-1-mediated adhesion between endothelial and mural cells is required for blood vessel maturation.
Garmy-Susini B, Jin H, Zhu Y, Sung RJ, Hwang R, Varner J
The Journal of clinical investigation 2005 Jun;115(6):1542-51
The Journal of clinical investigation 2005 Jun;115(6):1542-51
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band corresponding to the predicted size in kDa (+/-20%).
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 220, 112, 84, 47, 32, 26, 16.8
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 81
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human lateral ventricle shows nuclear positivity in glial cells.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.
