MA5-16429

antibody from Invitrogen Antibodies

Targeting: VCAM1 CD106

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-16429

Provider

Invitrogen Antibodies

Product name

VCAM-1 Monoclonal Antibody (1.G11B1)

Antibody type

Monoclonal

Antigen

Other

Description

Non-reducing conditions are recommended for Western blot applications.

Reactivity

Human, Porcine

Host

Mouse

Isotype

IgG

Antibody clone number

1.G11B1

Vial size

200 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

The pretubulysin-induced exposure of collagen is caused by endothelial cell retraction that results in an increased adhesion and decreased transmigration of tumor cells.

Schwenk R, Stehning T, Bischoff I, Ullrich A, Kazmaier U, Fürst R
Oncotarget 2017 Sep 29;8(44):77622-77633

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Adhesion of human hematopoietic progenitor cells to mesenchymal stromal cells involves CD44.

Wagner W, Wein F, Roderburg C, Saffrich R, Diehlmann A, Eckstein V, Ho AD
Cells, tissues, organs 2008;188(1-2):160-9

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Effects of vascular endothelial growth factor on the lymphocyte-endothelium interactions: identification of caveolin-1 and nitric oxide as control points of endothelial cell anergy.

Bouzin C, Brouet A, De Vriese J, Dewever J, Feron O
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 1;178(3):1505-11

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Immunological and ultrastructural characterization of endothelial cell cultures differentiated from human cord blood derived endothelial progenitor cells.

Neumüller J, Neumüller-Guber SE, Lipovac M, Mosgoeller W, Vetterlein M, Pavelka M, Huber J
Histochemistry and cell biology 2006 Dec;126(6):649-64

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Irradiation of mechanically-injured human arterial endothelial cells leads to increased gene expression and secretion of inflammatory and growth promoting cytokines.

Wondergem J, Wedekind LE, Bart CI, Chin A, van der Laarse A, Beekhuizen H
Atherosclerosis 2004 Jul;175(1):59-67

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Effect of maternal anti-HPA-1a antibodies and polyclonal IVIG on the activation status of vascular endothelial cells.

Radder CM, Beekhuizen H, Kanhai HH, Brand A
Clinical and experimental immunology 2004 Jul;137(1):216-22

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Human pulmonary valve endothelial cells express functional adhesion molecules for leukocytes.

Dvorin EL, Jacobson J, Roth SJ, Bischoff J
The Journal of heart valve disease 2003 Sep;12(5):617-24

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of VCAM-1 was performed using 70% confluent log phase HAEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with VCAM-1 Mouse monoclonal Antibody (Product # MA5-16429) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree Celsius and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2500 for 1 hour at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous and cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of KM-H2 cells stained with Mouse anti Human CD106

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 The enhanced expression of ICAM-1 or VCAM-1 is not responsible for the PT-triggered tumor cell adhesion (A/E) Confluent HUVECs were treated with PT (10, 30, 100 nM) or TNFalpha (10 ng/ml) for 12 h. The mRNA expression of ICAM-1 (A) or VCAM-1 (E) was analyzed by qPCR experiments. (B/F) Confluent HUVECs were treated with PT (10, 30, 100 nM) or TNFalpha (10 ng/ml) for 24 h. The total protein expression of ICAM-1 (B) or VCAM-1 (F) was determined by western blot analysis. (C/G) Confluent HUVECs were treated with PT (30, 100 nM) or TNFalpha (10 ng/ml) for 24 h. The cell surface expression of ICAM-1 (C) or VCAM-1 (G) was analyzed by flow cytometry. (D/H) Confluent HUVECs were treated with 30 nM PT for 24 h. An ICAM-1 blocking antibody (1, 5, 10 mug/ml) (D) or a VCAM-1 blocking antibody (1, 5, 10 mug/ml) (H) was added for the last 30 min of PT treatment. Fluorescence-labeled MDA cells were added and were allowed to adhere for 10 min. The amount of adherent MDA cells was determined by fluorescence measurements. (A-H) Data are expressed as mean +- SEM. A-C and E-H: n=3, D: n=5. *p 0.05 versus PT alone. TNFalpha was used as positive control and was not included into the statistical analyses.