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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- DM3521P - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#DM3521P, RRID:AB_1008448
- Product name
- anti VEGF-A (Isoform 165)
- Antibody type
- Monoclonal
- Antigen
- Recombinant Human VEGF165 protein (45 kDa) Cat.-No DA3514X
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 7G7
- Vial size
- 0.1 mg
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western analysis of recombinant human VEGF165, mouse VEGFG164 and human VEGF121 using a monoclonal mouse anti-human VEGF-A antibody Cat.-No DM3521P. There is no cross reactivity with mouse VEGF164.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- VEGF-A Sandwich-ELISA using the monoclonal mouse antihuman VEGF-A antibody Cat.-No DM3521P as capture antibody and recombinant human VEGF189 as standard. The Biotinylated monoclonal mouse anti-human VEGF-A antibody Cat.-No DM3521B was used for detection.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence staining of VEGF-A in a mixture of primary human dermal lymphatic endothelial cells (HDLEC) and human dermal normal fibroblasts with monoclonal mouse anti-Human VEGF-A Clone: 7G7 Cat.-No DM3521P.The cells were pretreated with 0,1% Triton X100. Control with goat anti-mouse ALEXA Flour 488, 1:600).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry with a paraffin-bedded section of human Glioblastoma tissue.
